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HRP Conjugated GST Rabbit Polyclonal Antibody

Usd: 339

Specification

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Catalog# 1006-3

HRP Conjugated GST Rabbit Polyclonal Antibody

Application

  • WB

  • ELISA

Reactivity

  • Species independent

Conjugation

  • HRP

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HRP Conjugated GST Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within GST protein.

Species Reactivity

Species independent

Validated Applications

WB, ELISA

Conjugation

HRP

RRID

AB_3068723

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000-1:10,000

  • ELISA

  • 1:10,000

Target

Function

The pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots, as well as for immunogen affinity purification of proteins expressed in E. coli.

Background References

暂无

Synonyms

Glutathione S Transferase antibody

Glutathione S-transferase class-mu 26 kDa isozyme antibody

GST antibody

Sj26 antigen antibody

SjGST antibody

Images

  • Western blot analysis of GST on different lysates with Rabbit anti-GST antibody (<a href="/products/1006-3" style="font-weight: bold;text-decoration: underline;">1006-3</a>) at 1/5,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: 293T transfected with GST cell lysate<br />Lane 3: 293T transfected with GST-tagged Histone H3.1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 18 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/1006-3" style="font-weight: bold;text-decoration: underline;">1006-3</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of GST on different lysates with Rabbit anti-GST antibody (1006-3) at 1/5,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: 293T transfected with GST cell lysate
    Lane 3: 293T transfected with GST-tagged Histone H3.1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 18 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1006-3) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation