AIM2 Recombinant Rabbit Monoclonal Antibody [PSH27-15]
Catalog# HA724676
AIM2 Recombinant Rabbit Monoclonal Antibody [PSH27-15]
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WB
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Human
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unconjugated
Overview
Product Name
AIM2 Recombinant Rabbit Monoclonal Antibody [PSH27-15]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human AIM2 aa 1-200.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 39 kDa
Positive Control
THP-1 treated with 200ng/mL hIFN-γ and 50ng/mL LPS for 24 hours add 300ng/mL BFA for last 20 hours cell lysate, HL-60 treated with 100ng/mL hIFN-γ for 24 hours cell lysate, Raji (Human Burkitt's lymphoma cell) cell lysate, U266 (Human multiple myeloma cell) cell lysate.
Conjugation
unconjugated
Clone Number
PSH27-15
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
Target
Function
Interferon-inducible protein AIM2 also known as absent in melanoma 2 or simply AIM2 is a protein that in humans is encoded by the AIM2 gene. AIM2 is a cytoplasmic sensor found in hematopoietic cells that recognizes the presence of double-stranded DNA (dsDNA) of microbial or host cellular origin. AIM2-like receptor (ALR) family was founded on AIM2 and now consists of four members in human genome. Activated AIM2 recruits apoptosis-associated speck-like protein containing a CARD (ASC), resulting in caspase-1 binding, and forming of AIM2 inflammasome. This signaling contributes to the defense against bacterial and viral DNA. The AIM2 inflammasome can also be an integral component of the AIM2-PANoptosome to drive PANoptosis.
Background References
1. Lee S et al. AIM2 forms a complex with pyrin and ZBP1 to drive PANoptosis and host defence. Nature. 2021 Sep
2. Zhang Y et al. AIM2 and Psoriasis. Front Immunol. 2023 Jan
Subcellular Location
Cytoplasm, Inflammasome, Nucleus.
UNIPROT
Synonyms
Absent in melanoma 2 antibody
AIM 2 antibody
Aim2 antibody
AIM2_HUMAN antibody
Interferon-inducible protein AIM2 antibody
OTTHUMP00000035296 antibody
PYHIN4 antibody
Images
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☑ Cell treatment (CT)
Western blot analysis of AIM2 on different lysates with Rabbit anti-AIM2 antibody (HA724676) at 1/5,000 dilution.
Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate
Lane 2: THP-1 treated with 200ng/mL hIFN-γ and 50ng/mL LPS for 24 hours, add 300ng/mL BFA for last 20 hours cell lysate
Lane 3: HL-60 (Human acute promyelocytic leukemia cell) cell lysate
Lane 4: HL-60 treated with 100ng/mL hIFN-γ for 24 hours cell lysate
Lane 5: Raji (Human Burkitt's lymphoma cell) cell lysate
Lane 6: U266 (Human multiple myeloma cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724676, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 39 kDa
Observed band size: 39-43 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
AIM2 Recombinant Rabbit Monoclonal Antibody [PSH27-15] - BSA and Azide free
Application: WB
Reactivity: Human
Conjugate: unconjugated
