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AIM2 Recombinant Rabbit Monoclonal Antibody [PSH27-15] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA752068

AIM2 Recombinant Rabbit Monoclonal Antibody [PSH27-15] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

AIM2 Recombinant Rabbit Monoclonal Antibody [PSH27-15] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human AIM2 aa 1-200.

Species Reactivity

Human

Validated Applications

WB

Target Molecular Weight

Predicted band size: 39 kDa

Positive Control

THP-1 treated with 200ng/mL hIFN-γ and 50ng/mL LPS for 24 hours add 300ng/mL BFA for last 20 hours cell lysate, HL-60 treated with 100ng/mL hIFN-γ for 24 hours cell lysate, Raji (Human Burkitt's lymphoma cell) cell lysate, U266 (Human multiple myeloma cell) cell lysate.

Conjugation

unconjugated

Clone Number

PSH27-15

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Interferon-inducible protein AIM2 also known as absent in melanoma 2 or simply AIM2 is a protein that in humans is encoded by the AIM2 gene. AIM2 is a cytoplasmic sensor found in hematopoietic cells that recognizes the presence of double-stranded DNA (dsDNA) of microbial or host cellular origin. AIM2-like receptor (ALR) family was founded on AIM2 and now consists of four members in human genome. Activated AIM2 recruits apoptosis-associated speck-like protein containing a CARD (ASC), resulting in caspase-1 binding, and forming of AIM2 inflammasome. This signaling contributes to the defense against bacterial and viral DNA. The AIM2 inflammasome can also be an integral component of the AIM2-PANoptosome to drive PANoptosis.

Background References

1. Lee S et al. AIM2 forms a complex with pyrin and ZBP1 to drive PANoptosis and host defence. Nature. 2021 Sep

2. Zhang Y et al. AIM2 and Psoriasis. Front Immunol. 2023 Jan

Subcellular Location

Cytoplasm, Inflammasome, Nucleus.

UNIPROT

SwissProt: O14862 Human

Synonyms

Absent in melanoma 2 antibody

AIM 2 antibody

Aim2 antibody

AIM2_HUMAN antibody

Interferon-inducible protein AIM2 antibody

OTTHUMP00000035296 antibody

PYHIN4 antibody

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />This data was developed using <a href="/products/HA724676" style="font-weight: bold;text-decoration: underline;">HA724676</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of AIM2 on different lysates with Rabbit anti-AIM2 antibody (<a href="/products/HA724676" style="font-weight: bold;text-decoration: underline;">HA724676</a>) at 1/5,000 dilution. <br /> <br />Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate <br />Lane 2: THP-1 treated with 200ng/mL hIFN-γ and 50ng/mL LPS for 24 hours, add 300ng/mL BFA for last 20 hours cell lysate <br />Lane 3: HL-60 (Human acute promyelocytic leukemia cell) cell lysate <br />Lane 4: HL-60 treated with 100ng/mL hIFN-γ for 24 hours cell lysate <br />Lane 5: Raji (Human Burkitt's lymphoma cell) cell lysate <br />Lane 6: U266 (Human multiple myeloma cell) cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 3 minutes; ECL: K1802 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724676" style="font-weight: bold;text-decoration: underline;">HA724676</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 39 kDa <br />Observed band size: 39-43 kDa

    ☑ Cell treatment (CT)

    This data was developed using HA724676, the same antibody clone in a different buffer formulation.
    Western blot analysis of AIM2 on different lysates with Rabbit anti-AIM2 antibody (HA724676) at 1/5,000 dilution.

    Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate
    Lane 2: THP-1 treated with 200ng/mL hIFN-γ and 50ng/mL LPS for 24 hours, add 300ng/mL BFA for last 20 hours cell lysate
    Lane 3: HL-60 (Human acute promyelocytic leukemia cell) cell lysate
    Lane 4: HL-60 treated with 100ng/mL hIFN-γ for 24 hours cell lysate
    Lane 5: Raji (Human Burkitt's lymphoma cell) cell lysate
    Lane 6: U266 (Human multiple myeloma cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 minutes; ECL: K1802

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724676, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 39 kDa
    Observed band size: 39-43 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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AIM2 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,FC

Reactivity: Human

Conjugate: unconjugated

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AIM2 Recombinant Rabbit Monoclonal Antibody [PSH27-15]

Application: WB

Reactivity: Human

Conjugate: unconjugated