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ATP2A1 / SERCA1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1803-34

ATP2A1 / SERCA1 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

ATP2A1 / SERCA1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetis peptide within mouse ATP2A1 aa 1-50 / 994.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 110 kDa

Positive Control

Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, Human skeletal muscle tissue lysate, rat skeletal muscle tissue, human fetal skeletal muscle tissue, mouse skeletal muscle tissue.

Conjugation

unconjugated

RRID

AB_3069230

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Key regulator of striated muscle performance by acting as the major Ca2+ ATPase responsible for the reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.

Background References

1. Tupling A R et al. Enhanced Ca2+ transport and muscle relaxation in skeletal muscle from sarcolipin-null mice. Am J Physiol 301:C841-C849 (2011).

2. Bal N C et al. Sarcolipin is a newly identified regulator of muscle-based thermogenesis in mammals. Nat Med 18:1575-1579 (2012).

Sequence Similarity

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.

Tissue Specificity

Skeletal muscle, fast twitch muscle (type II) fibers.

Subcellular Location

Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.

UNIPROT

SwissProt: O14983 Human

SwissProt: Q8R429 Mouse

SwissProt: Q64578 Rat

Synonyms

fast twitch skeletal muscle isoform antibody

AT2A1_HUMAN antibody

ATP2A antibody

ATP2A1 antibody

ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody

ATPase Ca++ transporting fast twitch 1 antibody

ATPase, Ca(2+)-transporting fast twitch 1 antibody

Calcium pump 1 antibody

Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody

Calcium-transporting ATPase sarcoplasmic reticulum type antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of ATP2A1 / SERCA1 on different lysates with Rabbit anti-ATP2A1 / SERCA1 antibody (<a href="/products/ER1803-34" style="font-weight: bold;text-decoration: underline;">ER1803-34</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse skeletal muscle tissue lysate<br />Lane 2: Mouse skeletal muscle tissue lysate (no heat)<br />Lane 3: Mouse cerebellum tissue lysate (no heat) (negative)<br />Lane 4: Mouse heart tissue lysate (negative)<br />Lane 5: Rat skeletal muscle tissue lysate<br />Lane 6: Rat cerebellum tissue lysate (negative)<br />Lane 7: Rat heart tissue lysate (negative)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 110 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 2 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER1803-34" style="font-weight: bold;text-decoration: underline;">ER1803-34</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of ATP2A1 / SERCA1 on different lysates with Rabbit anti-ATP2A1 / SERCA1 antibody (ER1803-34) at 1/2,000 dilution.

    Lane 1: Mouse skeletal muscle tissue lysate
    Lane 2: Mouse skeletal muscle tissue lysate (no heat)
    Lane 3: Mouse cerebellum tissue lysate (no heat) (negative)
    Lane 4: Mouse heart tissue lysate (negative)
    Lane 5: Rat skeletal muscle tissue lysate
    Lane 6: Rat cerebellum tissue lysate (negative)
    Lane 7: Rat heart tissue lysate (negative)

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 110 kDa
    Observed band size: 110 kDa

    Exposure time: 2 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-34) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ATP2A1 on different lysates using anti-ATP2A1 antibody at 1/500 dilution.<br /> <span style="font-weight: bold;">Positive control:</span><br /> Lane 1: Mouse skeletal muscle tissue <br /> Lane 2: Human skeletal muscle tissue

    Western blot analysis of ATP2A1 on different lysates using anti-ATP2A1 antibody at 1/500 dilution.
    Positive control:
    Lane 1: Mouse skeletal muscle tissue
    Lane 2: Human skeletal muscle tissue

  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Alternative Products

metafield image

ATP2A1 / SERCA1 Recombinant Rabbit Monoclonal Antibody [JE45-75]

Application: WB,IP,IHC-P

Reactivity: Human

Conjugate: unconjugated

Products with the same target and pathway

metafield image

ATP2A1 / SERCA1 Recombinant Rabbit Monoclonal Antibody [JE45-75]

Application: WB,IP,IHC-P

Reactivity: Human

Conjugate: unconjugated