ATP2A1 / SERCA1 Recombinant Rabbit Monoclonal Antibody [JE45-75]
Catalog# ET7109-43
ATP2A1 / SERCA1 Recombinant Rabbit Monoclonal Antibody [JE45-75]
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WB
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IP
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IHC-P
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Human
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unconjugated
Overview
Product Name
ATP2A1 / SERCA1 Recombinant Rabbit Monoclonal Antibody [JE45-75]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human SERCA1 ATPase aa 1-50 / 1,001.
Species Reactivity
Human
Validated Applications
WB, IP, IHC-P
Molecular Weight
Predicted band size: 110 kDa
Positive Control
Human fetal skeletal muscle tissue lysate, human fetal skeletal muscle tissue, human striated muscle tissue.
Conjugation
unconjugated
Clone Number
JE45-75
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IP
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1:10-1:50
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IHC-P
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1:50-1:400
Target
Function
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. There are 3 major domains on the cytoplasmic face of SERCA: the phosphorylation and nucleotide-binding domains, which form the catalytic site, and the actuator domain, which is involved in the transmission of major conformational changes. It seems that, in addition to the calcium-transporting properties, SERCA1 generates heat in some adipocytes and can improve cold tolerance in some wood frogs.
Background References
1. Tupling A R et al. Enhanced Ca2+ transport and muscle relaxation in skeletal muscle from sarcolipin-null mice. Am J Physiol 301:C841-C849 (2011).
2. Bal N C et al. Sarcolipin is a newly identified regulator of muscle-based thermogenesis in mammals. Nat Med 18:1575-1579 (2012).
Sequence Similarity
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
Tissue Specificity
Skeletal muscle, fast twitch muscle (type II) fibers.
Subcellular Location
Endoplasmic reticulum membrane, Sarcoplasmic reticulum membrane.
UNIPROT
Synonyms
fast twitch skeletal muscle isoform antibody
AT2A1_HUMAN antibody
ATP2A antibody
ATP2A1 antibody
ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
ATPase Ca++ transporting fast twitch 1 antibody
ATPase, Ca(2+)-transporting fast twitch 1 antibody
Calcium pump 1 antibody
Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody
Calcium-transporting ATPase sarcoplasmic reticulum type antibody
ExpandImages
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Western blot analysis of SERCA1 ATPase on human fetal skeletal muscle tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-SERCA1 ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-43) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-ATP2A1 / SERCA1 antibody (ET7109-43) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-43) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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