Alpha 1 Acid Glycoprotein Mouse Monoclonal Antibody [A4-G2-A3]
Catalog# EM1510-42
Alpha 1 Acid Glycoprotein Mouse Monoclonal Antibody [A4-G2-A3]
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WB
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IHC-P
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FC
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Human
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Rat
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Mouse
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unconjugated
Overview
Product Name
Alpha 1 Acid Glycoprotein Mouse Monoclonal Antibody [A4-G2-A3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human ORM1 aa 1-201 / 201.
Species Reactivity
Human, Rat (Predicted: Mouse)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 24 kDa
Positive Control
HepG2 cell lysate, HeLa cell lysate, THP-1 cell lysate, HCT 116 cell lysate, 293T cell lysate, NIH:OVCAR-3 cell lysate, human liver tissue lysate, rat liver tissue, human lung cancer tissue, human esophagus tissue, HepG2.
Conjugation
unconjugated
Clone Number
A4-G2-A3
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:50-1:100
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FC
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1:50-1:100
Target
Function
This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression. Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
Background References
1. Fitos I et al. Selective binding of imatinib to the genetic variants of human alpha1-acid glycoprotein. Biochim Biophys Acta 1760:1704-1712(2006).
2. Zsila F et al. The drug binding site of human alpha1-acid glycoprotein: insight from induced circular dichroism and electronic absorption spectra. Biochim Biophys Acta 1770:797-809(2007).
Sequence Similarity
Belongs to the calycin superfamily. Lipocalin family.
Tissue Specificity
Expressed by the liver and secreted in plasma.
Post-translational Modification
N-glycosylated. N-glycan heterogeneity at Asn-33: Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor).
Subcellular Location
Secreted.
Synonyms
A1AG1_HUMAN antibody
AGP 1 antibody
AGP A antibody
AGP antibody
AGP1 antibody
Alpha 1 acid glycoprotein antibody
Alpha-1-acid glycoprotein 1 antibody
alpha-1-AGP antibody
Epididymis secretory sperm binding protein Li 153w antibody
glycoprotein, alpha-1-acid, of serum antibody
ExpandImages
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Western blot analysis of Alpha 1 Acid Glycoprotein on different lysates with Mouse anti-Alpha 1 Acid Glycoprotein antibody (EM1510-42) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: THP-1 cell lysate (20 µg/Lane)
Lane 4: HCT 116 cell lysate (20 µg/Lane)
Lane 5: 293T cell lysate (20 µg/Lane)
Lane 6: NIH:OVCAR-3 cell lysate (20 µg/Lane)
Lane 7: Human liver tissue lysate (40 µg/Lane)
Predicted band size: 24 kDa
Observed band size: 37/45 kDa
Exposure time: 1 minute 59 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1510-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Alpha 1 Acid Glycoprotein antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Alpha 1 Acid Glycoprotein antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Alpha 1 Acid Glycoprotein antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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