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Bcl-2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1802-97

Bcl-2 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Bcl-2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human BCL-2 20-100 aa.

Species Reactivity

Human, Mouse

Validated Applications

WB, IF-Cell, FC

Molecular Weight

22/26 kDa

Positive Control

HL-60, mouse colon tissue lysate, LOVO, MCF-7, SH-SY-5Y.

Conjugation

unconjugated

RRID

AB_3069197

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:200-1:500

  • IF-Cell

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release.

Background References

1. Bruey J M et al. Bcl-2 and Bcl-XL regulate proinflammatory caspase-1 activation by interaction with NALP1. Cell 129:45-56 (2007).

2. Wei Y et al. JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy. Mol Cell 30:678-688 (2008).

Sequence Similarity

Belongs to the Bcl-2 family.

Tissue Specificity

Expressed in a variety of tissues.

Post-translational Modification

Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.

Subcellular Location

Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.

UNIPROT

SwissProt: P10415 Human

SwissProt: P10417 Mouse

Synonyms

Apoptosis regulator Bcl 2 antibody

Apoptosis regulator Bcl-2 antibody

Apoptosis regulator Bcl2 antibody

AW986256 antibody

B cell CLL/lymphoma 2 antibody

B cell leukemia/lymphoma 2 antibody

Bcl-2 antibody

Bcl2 antibody

BCL2_HUMAN antibody

C430015F12Rik antibody

Expand

Images

  • Western blot analysis of Bcl2 on different lysates using anti-Bcl2 antibody at 1/200 dilution.<br /> <span style="font-weight: bold;">Positive control:</span><br /> Lane 1: HL-60 <br /> Lane 2: Mouse colon<br />

    Western blot analysis of Bcl2 on different lysates using anti-Bcl2 antibody at 1/200 dilution.
    Positive control:
    Lane 1: HL-60
    Lane 2: Mouse colon

  • ICC staining Bcl2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Bcl2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Bcl2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Bcl2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Bcl2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Bcl2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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