Skip to content

Bcl-2 Recombinant Rabbit Monoclonal Antibody [SZ10-03]

Usd: 205

Specification

Bulk Order Quote | Customization Request

Catalog# ET1603-11

Bcl-2 Recombinant Rabbit Monoclonal Antibody [SZ10-03]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

  • FC

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Bcl-2 Recombinant Rabbit Monoclonal Antibody [SZ10-03]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Bcl-2 aa 40-90.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Molecular Weight

Predicted band size: 26 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, MCF7 cell lysate, HL-60 cell lysate, THP-1 cell lysate, A549, MCF-7, SH-SY5Y, human lymph nodes tissue, human kidney tissue, Jurkat.

Conjugation

unconjugated

Clone Number

SZ10-03

RRID

AB_2924968

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:800

  • IHC-P

  • 1:50-1:4,000

  • FC

  • 1:50-1:100

  • IP

  • Use at an assay dependent concentration.

Target

Function

Apoptosis is defined as a set of cascades which, when initiated, programs the cell to undergo lethal changes such as membrane blebbing, mitochondrial break down and DNA fragmentation. Bcl-2 is one among many key regulators of apoptosis, which are essential for proper development, tissue homeostasis, and protection against foreign pathogens. Human Bcl-2 is an anti-apoptotic, membrane-associated oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xl, Mcl-1, Bcl-w, and A1 or the death agonists Bax, Bak, Bik, Bad, and Bid. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phospho-rylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane. Mutations in the Bcl-2 gene can contribute to cancers where normal physiological cell death mechanisms are compromised by deregulation of the anti-apoptotic influence of Bcl-2.

Background References

1. Cao LH et al. Morphine, a potential antagonist of cisplatin cytotoxicity, inhibits cisplatin-induced apoptosis and suppression of tumor growth in nasopharyngeal carcinoma xenografts. Sci Rep 6:18706 (2016).

2. Chen B et al. Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells. Mol Med Rep 13:2527-35 (2016).

Sequence Similarity

Belongs to the Bcl-2 family.

Tissue Specificity

Expressed in a variety of tissues.

Post-translational Modification

Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.

Subcellular Location

Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.

UNIPROT

SwissProt: P10415 Human

Synonyms

Apoptosis regulator Bcl 2 antibody

Apoptosis regulator Bcl-2 antibody

Apoptosis regulator Bcl2 antibody

AW986256 antibody

B cell CLL/lymphoma 2 antibody

B cell leukemia/lymphoma 2 antibody

Bcl-2 antibody

Bcl2 antibody

BCL2_HUMAN antibody

C430015F12Rik antibody

Expand

Images

  • Western blot analysis of Bcl-2 on different lysates with Rabbit anti-Bcl-2 antibody (<a href="/products/ET1603-11" style="font-weight: bold;text-decoration: underline;">ET1603-11</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: MCF7 cell lysate (20 µg/Lane)<br />Lane 4: HL-60 cell lysate (20 µg/Lane)<br />Lane 5: THP-1 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 26 kDa<br />Observed band size: 26 kDa<br /><br />Exposure time: 24 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1603-11" style="font-weight: bold;text-decoration: underline;">ET1603-11</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Bcl-2 on different lysates with Rabbit anti-Bcl-2 antibody (ET1603-11) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: MCF7 cell lysate (20 µg/Lane)
    Lane 4: HL-60 cell lysate (20 µg/Lane)
    Lane 5: THP-1 cell lysate (20 µg/Lane)

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

    Exposure time: 24 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-11) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Bcl-2 on different lysates with Rabbit anti-Bcl-2 antibody (<a href="/products/ET1603-11" style="font-weight: bold;text-decoration: underline;">ET1603-11</a>) at 1/1,000 dilution.<br /><br />Lane 1: Hela-si NT cell lysate<br />Lane 2: Hela-si Bcl-2 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 26 kDa<br />Observed band size: 26 kDa<br /><br />Exposure time: 31 seconds; ECL: merk<br /><br />4-20% SDS-PAGE gel.<br /><br /><a href="/products/ET1603-11" style="font-weight: bold;text-decoration: underline;">ET1603-11</a> was shown to specifically react with Bcl-2 in Hela-si NT cells. Weakened band was observed when Hela-si Bcl-2 sample was tested. Hela-si NT and Hela-si Bcl-2 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1603-11" style="font-weight: bold;text-decoration: underline;">ET1603-11</a>, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, <a href="/products/ET1601-4" style="font-weight: bold;text-decoration: underline;">ET1601-4</a>, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Bcl-2 on different lysates with Rabbit anti-Bcl-2 antibody (ET1603-11) at 1/1,000 dilution.

    Lane 1: Hela-si NT cell lysate
    Lane 2: Hela-si Bcl-2 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

    Exposure time: 31 seconds; ECL: merk

    4-20% SDS-PAGE gel.

    ET1603-11 was shown to specifically react with Bcl-2 in Hela-si NT cells. Weakened band was observed when Hela-si Bcl-2 sample was tested. Hela-si NT and Hela-si Bcl-2 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1603-11, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • ICC staining of Bcl-2 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1603-11" style="font-weight: bold;text-decoration: underline;">ET1603-11</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Bcl-2 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-11, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of Bcl-2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1603-11" style="font-weight: bold;text-decoration: underline;">ET1603-11</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Bcl-2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-11, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of Bcl-2 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1603-11" style="font-weight: bold;text-decoration: underline;">ET1603-11</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Bcl-2 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-11, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Flow cytometric analysis of Bcl-2 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET1603-11" style="font-weight: bold;text-decoration: underline;">ET1603-11</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Bcl-2 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1603-11, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-Bcl-2 antibody (<a href="/products/ET1603-11" style="font-weight: bold;text-decoration: underline;">ET1603-11</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1603-11" style="font-weight: bold;text-decoration: underline;">ET1603-11</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-Bcl-2 antibody (ET1603-11) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-11) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-Bcl-2 antibody (<a href="/products/ET1603-11" style="font-weight: bold;text-decoration: underline;">ET1603-11</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1603-11" style="font-weight: bold;text-decoration: underline;">ET1603-11</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-Bcl-2 antibody (ET1603-11) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-11) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

Phospho-Bcl-2 (S70) Recombinant Rabbit Monoclonal Antibody [PSH11-02] - BSA and Azide free

Application: WB,IF-Cell,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2 Recombinant Rabbit Monoclonal Antibody [JF104-8]

Application: WB,IF-Cell,IF-Tissue,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Bcl-2 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2 Recombinant Mouse Monoclonal Antibody [9F3-R]

Application: WB,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2 Recombinant Rabbit Monoclonal Antibody

Application: mIHC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2 Recombinant Rabbit Monoclonal Antibody [SZ10-03] - BSA and Azide free

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2 Mouse Monoclonal Antibody [9F1]

Application: WB,IHC-P

Reactivity: Human

Conjugate: unconjugated

metafield image

Phospho-Bcl-2 (S70) Recombinant Rabbit Monoclonal Antibody [PSH11-02]

Application: WB,IF-Cell,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2 Mouse Monoclonal Antibody [9F3]

Application: WB,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2 Recombinant Rabbit Monoclonal Antibody [JF104-8] - BSA and Azide free

Application: WB,IF-Cell,IF-Tissue,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Bcl-2 Recombinant Mouse Monoclonal Antibody [9F3-R] - BSA and Azide free

Application: WB,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2 Recombinant Rabbit Monoclonal Antibody [PSH09-49] - BSA and Azide free

Application: WB,IF-Cell,IHC-P,FC,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Bcl-2 Recombinant Rabbit Monoclonal Antibody [PD01-41]

Application: WB,IHC-P,IF-Cell,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Bcl-2 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,FC

Reactivity: Human,Mouse

Conjugate: unconjugated

metafield image

Bcl-2 Mouse Monoclonal Antibody [F7-1-A1]

Application: WB,IF-Cell,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

FITC Conjugated Bcl-2 Recombinant Rabbit Monoclonal Antibody [JF104-8]

Application: FC

Reactivity: Human,Mouse

Conjugate: FITC

metafield image

Bcl-2 Recombinant Rabbit Monoclonal Antibody [PSH09-49]

Application: WB,IF-Cell,IHC-P,FC,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated