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CA125/MUC16 Recombinant Rabbit Monoclonal Antibody [PD01-13]

Usd: 385

Specification

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Catalog# HA721217

CA125/MUC16 Recombinant Rabbit Monoclonal Antibody [PD01-13]

Application

  • IHC-P

  • WB

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

CA125/MUC16 Recombinant Rabbit Monoclonal Antibody [PD01-13]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide.

Species Reactivity

Human

Validated Applications

IHC-P, WB, IF-Tissue

Molecular Weight

Predicted band size: 1,519 kDa

Positive Control

Human ovarian serous carcinoma tissue, human endometrium tissue, human ovary cancer tissue, NIH:OVCAR-3 cell lysate, HeLa cell lysate.

Conjugation

unconjugated

Clone Number

PD01-13

RRID

AB_3072337

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:1,000-1:2,000

  • WB

  • 1:1,000

  • IF-Tissue

  • 1:1,000

Target

Function

Mucin-16 (MUC-16) also known as Ovarian cancer-related tumor marker CA125 is a protein that in humans is encoded by the MUC16 gene. MUC-16 is a member of the mucin family glycoproteins. MUC-16 has found application as a tumor marker or biomarker that may be elevated in the blood of some patients with specific types of cancers, most notably ovarian cancer, or other conditions that are benign. MUC16 is a component of the ocular surface (including the cornea and conjunctiva), the respiratory tract and the female reproductive tract epithelia. Since MUC16 is highly glycosylated it creates a hydrophilic environment that acts as a lubricating barrier against foreign particles and infectious agents on the apical membrane of epithelial cells. Also, the cytoplasmic tail of MUC16 has been shown to interact with cytoskeleton by binding members of the ERM protein family. The expression of mucin 16 has been shown to be altered in dry eye, cystic fibrosis, and several types of cancers.

Background References

1. Giamougiannis P et al. The evolving role of MUC16 (CA125) in the transformation of ovarian cells and the progression of neoplasia. Carcinogenesis. 2021 Apr

2. Wang F et al. MUC16 promotes EOC proliferation by regulating GLUT1 expression. J Cell Mol Med. 2021 Mar

Subcellular Location

Cell membrane, secreted, extracellular space.

UNIPROT

SwissProt: Q8WXI7 Human

Synonyms

CA 125 antibody

CA-125 antibody

CA125 antibody

CA125 ovarian cancer antigen antibody

Cancer antigen 125 antibody

FLJ14303 antibody

MUC 16 antibody

MUC-16 antibody

MUC16 antibody

MUC16_HUMAN antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma tissue with Rabbit anti-CA125/MUC16 antibody (<a href="/products/HA721217" style="font-weight: bold;text-decoration: underline;">HA721217</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721217" style="font-weight: bold;text-decoration: underline;">HA721217</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma tissue with Rabbit anti-CA125/MUC16 antibody (HA721217) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721217) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-CA125/MUC16 antibody (<a href="/products/HA721217" style="font-weight: bold;text-decoration: underline;">HA721217</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721217" style="font-weight: bold;text-decoration: underline;">HA721217</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-CA125/MUC16 antibody (HA721217) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721217) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-CA125/MUC16 antibody (<a href="/products/HA721217" style="font-weight: bold;text-decoration: underline;">HA721217</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721217" style="font-weight: bold;text-decoration: underline;">HA721217</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-CA125/MUC16 antibody (HA721217) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721217) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-CA125/MUC16 antibody (<a href="/products/HA721217" style="font-weight: bold;text-decoration: underline;">HA721217</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721217" style="font-weight: bold;text-decoration: underline;">HA721217</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-CA125/MUC16 antibody (HA721217) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721217) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of CA125/MUC16 on different lysates with Rabbit anti-CA125/MUC16 antibody (<a href="/products/HA721217" style="font-weight: bold;text-decoration: underline;">HA721217</a>) at 1/1,000 dilution.<br /><br />Lane 1: NIH:OVCAR-3 cell lysate<br />Lane 2: HeLa cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 1519 kDa<br /><br /><br />Exposure time: 25 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721217" style="font-weight: bold;text-decoration: underline;">HA721217</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CA125/MUC16 on different lysates with Rabbit anti-CA125/MUC16 antibody (HA721217) at 1/1,000 dilution.

    Lane 1: NIH:OVCAR-3 cell lysate
    Lane 2: HeLa cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 1519 kDa


    Exposure time: 25 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721217) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: ovarian serous carcinoma<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/1,000

    Application: IF-Tissue

    Species: Human

    Site: ovarian serous carcinoma

    Sample: Paraffin-embedded section

    Antibody concentration: 1/1,000

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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