Human CA125/MUC16 enzyme-linked immunoassay kit
Catalog# EH0282
Human CA125/MUC16 enzyme-linked immunoassay kit
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ELISA
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Human
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15.63~1000 pg/ml
Overview
Product Name
Human CA125/MUC16 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.57 pg/ml (50 μl)；3.91 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0640 | 0.0610 | 0.0625 | |
| 15.63 | 0.1398 | 0.1348 | 0.1373 | 0.0748 |
| 31.25 | 0.2330 | 0.2304 | 0.2317 | 0.1692 |
| 62.50 | 0.4281 | 0.4103 | 0.4192 | 0.3567 |
| 125.00 | 0.8426 | 0.7343 | 0.7885 | 0.7260 |
| 250.00 | 1.5720 | 1.3590 | 1.4655 | 1.4030 |
| 500.00 | 2.8420 | 2.5660 | 2.7040 | 2.6415 |
| 1000.00 | 4.0496 | 3.9340 | 3.9918 | 3.9293 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 10.9 | 71.5 | 218.7 | 10.9 | 80.9 | 263.6 |
| Standard Deviation | 0.8 | 5.4 | 15.3 | 0.7 | 5.7 | 15.6 |
| Coefficient of Variation (%) | 7.4 | 7.5 | 7.0 | 6.5 | 7.1 | 5.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CA125/MUC16 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 76% to 108% with an overall mean recovery of 87%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 38.89-663.06 | 100 | 124.51 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human CA125/MUC16 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃