Human NG2/MCSP/CSPG4 enzyme-linked immunoassay kit(one step)
Catalog# EHY0162
Human NG2/MCSP/CSPG4 enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.69~500 ng/ml
Overview
Product Name
Human NG2/MCSP/CSPG4 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.004 ng/ml (50 μl)；0.21 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0144 | 0.0150 | 0.0147 | |
| 0.69 | 0.0266 | 0.0269 | 0.0268 | 0.0121 |
| 2.06 | 0.0461 | 0.0456 | 0.0459 | 0.0312 |
| 6.17 | 0.1073 | 0.1186 | 0.1130 | 0.0983 |
| 18.52 | 0.2726 | 0.3004 | 0.2865 | 0.2718 |
| 55.56 | 0.8309 | 0.8644 | 0.8477 | 0.8330 |
| 166.67 | 2.0830 | 2.1660 | 2.1245 | 2.1098 |
| 500.00 | 3.8140 | 3.9010 | 3.8575 | 3.8428 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.7 | 8.6 | 30.9 | 1.6 | 8.9 | 31.6 |
| Standard Deviation | 0.1 | 0.4 | 2.0 | 0.1 | 0.6 | 1.8 |
| Coefficient of Variation (%) | 5.7 | 4.4 | 6.5 | 6.0 | 6.8 | 5.7 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 NG2/MCSP/CSPG4，未加人 NG2/MCSP/CSPG4 的血清作为本底，计算回收率。回收率的范围从 99 % 至 117 %，平均回收率为 108 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 59.50-274.06 | 100 | 129.52 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
4732461B14Rik
AN2
AN2 proteoglycan
Chondroitin sulfate proteoglycan 4 (melanoma-associated)
Chondroitin sulfate proteoglycan 4
Chondroitin sulfate proteoglycan NG2
Cspg4
Cspg4 chondroitin sulfate proteoglycan 4
CSPG4_HUMAN
HMW-MAA
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