Human NG2/MCSP/CSPG4 enzyme-linked immunoassay kit(one step)
Catalog# EHY0162
Human NG2/MCSP/CSPG4 enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.69~500 ng/ml
Overview
Product Name
Human NG2/MCSP/CSPG4 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.004 ng/ml (50 μl)；0.21 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0144 | 0.0150 | 0.0147 | |
| 0.69 | 0.0266 | 0.0269 | 0.0268 | 0.0121 |
| 2.06 | 0.0461 | 0.0456 | 0.0459 | 0.0312 |
| 6.17 | 0.1073 | 0.1186 | 0.1130 | 0.0983 |
| 18.52 | 0.2726 | 0.3004 | 0.2865 | 0.2718 |
| 55.56 | 0.8309 | 0.8644 | 0.8477 | 0.8330 |
| 166.67 | 2.0830 | 2.1660 | 2.1245 | 2.1098 |
| 500.00 | 3.8140 | 3.9010 | 3.8575 | 3.8428 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.7 | 8.6 | 30.9 | 1.6 | 8.9 | 31.6 |
| Standard Deviation | 0.1 | 0.4 | 2.0 | 0.1 | 0.6 | 1.8 |
| Coefficient of Variation (%) | 5.7 | 4.4 | 6.5 | 6.0 | 6.8 | 5.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human NG2/MCSP/CSPG4 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 99% to 117% with an overall mean recovery of 108%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 59.50-274.06 | 100 | 129.52 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of NG2/MCSP/CSPG4 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
4732461B14Rik
AN2
AN2 proteoglycan
Chondroitin sulfate proteoglycan 4 (melanoma-associated)
Chondroitin sulfate proteoglycan 4
Chondroitin sulfate proteoglycan NG2
Cspg4
Cspg4 chondroitin sulfate proteoglycan 4
CSPG4_HUMAN
HMW-MAA
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