Human IFN-α enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

96T

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Catalog# EH0085

Human IFN-α enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

0.41~300 pg/ml

Overview

Product Name

Human IFN-α enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.03 pg/ml (50 μl) ；0.24 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0187	0.0181	0.0184
0.41	0.0407	0.0408	0.0408	0.0224
1.23	0.0767	0.0753	0.0760	0.0576
3.70	0.1796	0.1758	0.1777	0.1593
11.11	0.4858	0.4840	0.4849	0.4665
33.33	1.2260	1.2340	1.2300	1.2116
100.00	2.5610	2.5950	2.5780	2.5596
300.00	4.2605	4.2605	4.2605	4.2421

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	11.4	51.2	155.6	12.0	57.0	175
Standard Deviation	0.5	3.8	9.8	0.7	1.8	4.2
Coefficient of Variation (%)	4.4	7.3	6.3	5.3	3.1	2.4

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human IFN-alpha into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 128% with an overall mean recovery of 98%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.-0.9	31.3	0.21

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: P01562 Human

Alias

IFL

IFN alpha

IFN alpha 1/13

IFN alpha 1b

IFN alphaD

IFN

IFN-alpha-1/13

IFNA@

IFNA1

IFNA1_HUMAN

Expand

Function

There are 3 major classes of interferons (IFNs): Type I, Type II and Type III. Interferon alpha (IFN-alpha ), along with IFN-beta, IFN-δ, IFN-epsilon, IFN-kappa, IFN-omega and IFN-tau are all Type I IFNs. The sole type II IFN is IFN-gamma. Type III IFNs include IFN-lambda 1, IFN-lambda 2, IFN-lambda 3 and IFN-lambda 4. As a part of the innate immune response, Type I IFNs are rapidly induced in response to viral nucleic acids such as double stranded DNA or RNA (dsDNA, dsRNA) and single stranded RNA (ssRNA), viral glycoproteins, microbial cytosinephosphate-guanosine (CpG) DNA, DNA damage, and chromosomal instability. IFN-alpha subtypes are well described. There are 15 human IFN-alpha subtypes with 80% amino acid identity. The number of IFN-alpha subtypes varies by species with 6 equine subtypes, 17 porcine subtypes, 14 bovine subtypes, and 9 canine subtypes known currently. Human IFN-alpha subtypes include: IFN-alpha 1a, IFN-alpha 1b, IFN-alpha 2a, IFN-alpha 2b, IFN-alpha 4a, IFN-alpha 4b, IFN-alpha 5, IFN-alpha 6 IFN-alpha 7, IFN-alpha 8, IFN-alpha 10, IFN-alpha 14, IFN-alpha 16, IFN-alpha 17, and IFN-alpha 21. Although there is one known heterodimeric IFN-alpha receptor (IFN-alpha R, described below), each IFN-alpha subtype has been correlated with differing biological activities. Variability in biological responses can be attributed to differences in binding affinity and duration, receptor density, feedback responses and intracellular characteristics. IFN-alpha responses have been described as robust, especially in the context of viral infection responsiveness by all cells or tunable in a cell type specific manner. IFN-alpha signaling is well characterized. IFN-alpha is a ligand for IFN alpha R, which includes two subunits IFN alpha R1 and IFN alpha R2. IFN-alpha ligand binding to the ubiquitously expressed IFN alpha R1 triggers a conformational change which allows for the heterodimerization of IFN alpha R1 and IFN alpha R2. Heterodimerization results in the cross phosphorylation of the Janus-activated Kinase 1 (JAK1) on IFN alpha R2 and tyrosine kinase 2 (TYK2) on IFN alpha R1 respectively, as well as the intracellular domain of IFN alpha R1 and IFN alpha 2. The transcription factors Signal transducer and activator of transcription (STAT) 1 and 2 are subsequently recruited to IFN alpha R via their Src homology 2 (SH2) domain and phosphorylated. In the canonical IFN-alpha signaling pathway, phosphoSTAT1/STAT2 heterodimers associate with Interferon Regulatory Factor 9 (IRF9) to form Interferon Stimulated Gene Factor 3 (ISGF3), which translocates into the nucleus to bind to gamma-activated sequences (GASs) or interferon- stimulated response elements (ISREs). This stimulates the transcription of interferonstimulated genes (ISGs). STAT 2 homodimers and monomers can also associate with IRF-9 to form ISGF3-like complex, which also binds to ISRE to stimulate the transcription of ISGs. Although IFN-alpha is most commonly associated with viral infections, it has been associated with other pathological events. Type 1 interferons represent a standard of care for suppressing Hepatitis B (HBV) or C (HCV). However, it has been associated with neuropsychiatric symptoms such as depression, anhedonia, anxiety and cognitive impairment. The role of IFN-alpha in cancer is complex as well. For example, in the context of inflammatory breast cancer, IFN-alpha is upregulated. Although IFN-alpha has been correlated with cellular senescence and apoptosis, some subtypes have been correlated with increased cellular migration and drug resistance.

Human IFN-α enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Alias

Function

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