Human CCL13/MCP-4 enzyme-linked immunoassay kit
Catalog# EH0199
Human CCL13/MCP-4 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
0.69~500 pg/ml
Overview
Product Name
Human CCL13/MCP-4 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.03 pg/ml (50 μl)；0.18 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0085 | 0.0090 | 0.0088 | |
| 0.69 | 0.0257 | 0.0247 | 0.0252 | 0.0165 |
| 2.06 | 0.0596 | 0.0624 | 0.0610 | 0.0523 |
| 6.17 | 0.1737 | 0.1756 | 0.1747 | 0.1659 |
| 18.52 | 0.5100 | 0.4768 | 0.4934 | 0.4847 |
| 55.56 | 1.3790 | 1.2690 | 1.3240 | 1.3153 |
| 166.67 | 3.0120 | 2.9570 | 2.9845 | 2.9758 |
| 500.00 | 4.2130 | 4.3809 | 4.2970 | 4.2882 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 11.6 | 60.1 | 194.9 | 10.7 | 53.7 | 177.4 |
| Standard Deviation | 0.4 | 1.9 | 6.6 | 0.6 | 2.3 | 9.1 |
| Coefficient of Variation (%) | 3.1 | 3.2 | 3.4 | 5.2 | 4.3 | 5.2 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 CCL13/MCP-4，未加人 CCL13/MCP-4 的血清作为本底，计算回收率。回收率的范围从 80 % 至 120 %，平均回收率为 100 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 22.30-140.00 | 100 | 75.86 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C-C motif chemokine 13
CCL13
CCL13_HUMAN
Chemokine (C C motif) ligand 13
CK beta 10
CK-beta-10
CKb10
MCP-4
MGC17134
Monocyte chemoattractant protein 4
ExpandFunction
人 MCP-4，也称为 CCL13，编码 98 个氨基酸 (aa) 的前体蛋白，具有 23 个氨基酸的疏水信号肽，该信号肽被切割后产生 8 kDa、75 个氨基酸的成熟 MCP-4。成熟的 MCP-4 缺乏任何潜在的 N-糖基化位点，并与其他人类 MCP 蛋白共享焦谷氨酸脯氨酸基序。