Human CD48 enzyme-linked immunoassay kit
Catalog# EH0119
Human CD48 enzyme-linked immunoassay kit
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ELISA
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Human
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1.37~1000 pg/ml
Overview
Product Name
Human CD48 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.25 pg/ml (50 μl)；1.05 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0137 | 0.0135 | 0.0136 | |
| 1.37 | 0.0207 | 0.0203 | 0.0205 | 0.0069 |
| 4.12 | 0.0328 | 0.0325 | 0.0327 | 0.0191 |
| 12.35 | 0.0735 | 0.0744 | 0.0740 | 0.0604 |
| 37.04 | 0.1995 | 0.1974 | 0.1985 | 0.1849 |
| 111.11 | 0.5747 | 0.5406 | 0.5577 | 0.5441 |
| 333.33 | 1.4730 | 1.6080 | 1.5405 | 1.5269 |
| 1000.00 | 3.3010 | 3.2540 | 3.2775 | 3.2639 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 22.9 | 105.7 | 348.3 | 22.5 | 109.1 | 338.0 |
| Standard Deviation | 0.7 | 3.3 | 12.2 | 1.0 | 3.3 | 12.2 |
| Coefficient of Variation (%) | 3.2 | 3.1 | 3.5 | 4.4 | 3.0 | 3.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD48 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 109% with an overall mean recovery of 93%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2395.44-4374.87 | 100 | 3246.68 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD48 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD48 antigen
CD48 molecule
CD48
hCD48
SLAMF2
BCM1 surface antigen
BCM1
BCM1Leukocyte antigen MEM-102
BLAST
BLAST1
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