Human MMP-9 enzyme-linked immunoassay kit
Catalog# EH0020
Human MMP-9 enzyme-linked immunoassay kit
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ELISA
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Human
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13.72~10000 pg/ml
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unconjugated
Overview
Product Name
Human MMP-9 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.02 pg/ml (50 μl) ； 5.99 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0406 | 0.0373 | 0.0390 | |
| 13.72 | 0.0554 | 0.0543 | 0.0549 | 0.0159 |
| 41.15 | 0.0822 | 0.0835 | 0.0829 | 0.0439 |
| 123.46 | 0.1751 | 0.1707 | 0.1729 | 0.1340 |
| 370.37 | 0.4324 | 0.4273 | 0.4299 | 0.3909 |
| 1111.11 | 1.0860 | 1.0930 | 1.0895 | 1.0506 |
| 3333.33 | 2.4790 | 2.5010 | 2.4900 | 2.4511 |
| 10000.00 | 3.9685 | 4.0557 | 4.0121 | 3.9732 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 225.2 | 1135.8 | 3363.1 | 234.0 | 1080.9 | 3328.7 |
| Standard Deviation | 6.4 | 58.6 | 182.6 | 12.3 | 16.5 | 111.9 |
| Coefficient of Variation (%) | 2.8 | 5.2 | 5.4 | 5.3 | 1.5 | 3.4 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人MMP-9，未加人 MMP-9 的血清作为本底，计算回收率。回收率的范围从 88 % 至 112 %，平均回收率为 103 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 520.39-1780.56 | 100 | 1106.20 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
82 kDa matrix metalloproteinase-9
92 kDa gelatinase
92 kDa type IV collagenase
CLG 4B
CLG4B
Collagenase Type 4 beta
Collagenase type IV 92 KD
EC 3.4.24.35
Gelatinase 92 KD
Gelatinase B
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