Human CD155/PVR(Poliovirus Receptor) enzyme-linked immunoassay kit(one step)
Catalog# EHY0155
Human CD155/PVR(Poliovirus Receptor) enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.41~300 ng/ml
Overview
Product Name
Human CD155/PVR(Poliovirus Receptor) enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.02 ng/ml (50 μl)；0.11 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0084 | 0.0085 | 0.0085 | |
| 0.41 | 0.0244 | 0.0238 | 0.0241 | 0.0157 |
| 1.23 | 0.0572 | 0.0551 | 0.0562 | 0.0477 |
| 3.70 | 0.1522 | 0.1557 | 0.1540 | 0.1455 |
| 11.11 | 0.4902 | 0.4839 | 0.4871 | 0.4786 |
| 33.33 | 1.4070 | 1.4170 | 1.4120 | 1.4036 |
| 100.00 | 3.0310 | 3.1030 | 3.0670 | 3.0586 |
| 300.00 | 3.8790 | 3.9340 | 3.9065 | 3.8981 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 6.0 | 32.6 | 126.4 | 5.3 | 30.0 | 119.7 |
| Standard Deviation | 0.2 | 1.3 | 4.0 | 0.2 | 0.5 | 1.7 |
| Coefficient of Variation (%) | 3.3 | 4.0 | 3.1 | 3.1 | 1.8 | 1.4 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 CD155/PVR(Poliovirus Receptor)，未加人 CD155/PVR(Poliovirus Receptor) 的血清作为本底，计算回收率。回收率的范围从 82 % 至 103 %，平均回收率为 91%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 571.24-1084.17 | 100 | 904.22 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD155
CD155 antigen
FLJ25946
HVED
mE4
NECL 5
Necl-5
Necl5
Nectin like 5
Nectin like protein 5
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