Human CXCL13/BCA-1 enzyme-linked immunoassay kit
Catalog# EH0172
Human CXCL13/BCA-1 enzyme-linked immunoassay kit
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ELISA
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Human
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7.81~500 pg/ml
Overview
Product Name
Human CXCL13/BCA-1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.56 pg/ml (50 μl)；5.32 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0076 | 0.0074 | 0.0075 | |
| 7.81 | 0.0260 | 0.0265 | 0.0263 | 0.0188 |
| 15.63 | 0.0515 | 0.0509 | 0.0512 | 0.0437 |
| 31.25 | 0.1190 | 0.1180 | 0.1185 | 0.1110 |
| 62.50 | 0.2836 | 0.2700 | 0.2768 | 0.2693 |
| 125.00 | 0.7076 | 0.6990 | 0.7033 | 0.6958 |
| 250.00 | 1.8240 | 1.8140 | 1.8190 | 1.8115 |
| 500.00 | 3.5660 | 3.6350 | 3.6005 | 3.5930 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 19.3 | 78.3 | 213.9 | 18.5 | 76.1 | 216.7 |
| Standard Deviation | 0.7 | 2.7 | 9.6 | 0.7 | 2.3 | 7.1 |
| Coefficient of Variation (%) | 3.4 | 3.4 | 4.5 | 3.8 | 3.0 | 3.3 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 CXCL13/BCA-1，未加人 CXCL13/BCA-1 的血清作为本底，计算回收率。回收率的范围从 80 % 至 120 %，平均回收率为 96 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 28.44-275.19 | 100 | 71.8 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
ANGIE
ANGIE2
B cell attracting chemokine 1
B cell-attracting chemokine 1
B lymphocyte chemoattractant
B-cell chemoattractant
B-cell-attracting chemokine 1
B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
BCA-1
BCA1
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