Human MICB enzyme-linked immunoassay kit
Catalog# EH0127
Human MICB enzyme-linked immunoassay kit
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ELISA
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Human
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6.86~5000 pg/ml
Overview
Product Name
Human MICB enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.09 pg/ml (50 μl)；6.68 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0208 | 0.0166 | 0.0187 | |
| 6.86 | 0.0321 | 0.0300 | 0.0311 | 0.0124 |
| 20.58 | 0.0507 | 0.0474 | 0.0491 | 0.0304 |
| 61.73 | 0.0996 | 0.0959 | 0.0978 | 0.0791 |
| 185.19 | 0.2567 | 0.2492 | 0.2530 | 0.2343 |
| 555.56 | 0.7067 | 0.7088 | 0.7078 | 0.6891 |
| 1666.67 | 2.0300 | 1.9550 | 1.9925 | 1.9738 |
| 5000.00 | 3.8400 | 3.7960 | 3.8180 | 3.7993 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 104.3 | 521.4 | 1862.1 | 99.0 | 528.2 | 1622.6 |
| Standard Deviation | 4.2 | 23.5 | 101.7 | 8.2 | 28.2 | 62.3 |
| Coefficient of Variation (%) | 4.1 | 4.5 | 5.5 | 5.2 | 5.3 | 3.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human MICB into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from84% to 108% with an overall mean recovery of 96%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2.38-168.59 | 100 | 45.33 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD137/4-1BB/TNFRSF9 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
MHC class I chain-related protein A
MHC class I chain-related protein B
MHC class I polypeptide related sequence A
MHC class I polypeptide related sequence B
MHC class I polypeptide-related sequence A
MIC-A
micA
MICA_HUMAN
MICB