Human Hemoglobin subunit alpha/HBA1 enzyme-linked immunoassay kit
Catalog# EH0247
Human Hemoglobin subunit alpha/HBA1 enzyme-linked immunoassay kit
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ELISA
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Human
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68.59~50000 pg/ml
Overview
Product Name
Human Hemoglobin subunit alpha/HBA1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
9.11 pg/ml (50 μl)；28.56 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0079 | 0.0078 | 0.0079 | |
| 68.59 | 0.0139 | 0.0142 | 0.0141 | 0.0062 |
| 205.76 | 0.0293 | 0.0308 | 0.0301 | 0.0222 |
| 617.28 | 0.0938 | 0.0880 | 0.0909 | 0.0831 |
| 1851.85 | 0.3062 | 0.2938 | 0.3000 | 0.2922 |
| 5555.56 | 1.0950 | 1.1060 | 1.1005 | 1.0927 |
| 16666.67 | 2.5210 | 2.4460 | 2.4835 | 2.4757 |
| 50000.00 | 3.9190 | 3.8470 | 3.8830 | 3.8752 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 945.9 | 4900.6 | 14097.9 | 1278.0 | 5808.4 | 16647.0 |
| Standard Deviation | 72.9 | 355.0 | 1048.4 | 88.4 | 144.2 | 638.3 |
| Coefficient of Variation (%) | 7.7 | 7.2 | 7.4 | 6.9 | 2.5 | 3.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Hemoglobin subunit alpha/HBA1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 94% to 122% with an overall mean recovery of 104%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 78.22-476.01 | 100 | 193.92 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Hemoglobin subunit alpha/HBA1 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃