Human DLL1 enzyme-linked immunoassay kit
Catalog# EH0323
Human DLL1 enzyme-linked immunoassay kit
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ELISA
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Human
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156.25 ~10000 pg/ml
Overview
Product Name
Human DLL1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
11.07 pg/ml (50 μl)；39.33 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0262 | 0.0257 | 0.0260 | |
| 156.25 | 0.0730 | 0.0700 | 0.0715 | 0.0456 |
| 312.50 | 0.1135 | 0.1163 | 0.1149 | 0.0890 |
| 625.00 | 0.2173 | 0.2206 | 0.2190 | 0.1930 |
| 1250.00 | 0.4625 | 0.4238 | 0.4432 | 0.4172 |
| 2500.00 | 0.9122 | 0.9081 | 0.9102 | 0.8842 |
| 5000.00 | 1.7470 | 1.7180 | 1.7325 | 1.7066 |
| 10000.00 | 2.9250 | 2.9760 | 2.9505 | 2.9246 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 127.8 | 939.6 | 3575.8 | 140.2 | 929.3 | 3044.1 |
| Standard Deviation | 9.2 | 40.6 | 136.8 | 7.1 | 53.9 | 222.1 |
| Coefficient of Variation (%) | 7.2 | 4.3 | 3.8 | 2.4 | 2.4 | 3.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human DLL1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 110% with an overall mean recovery of 95%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2923.91-4581.01 | 100 | 3687.35 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human DLL1 in this assay. No medical histories were available for the donors.n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃