Human CCL3/MIP-1 enzyme-linked immunoassay kit
Catalog# EH0146
Human CCL3/MIP-1 enzyme-linked immunoassay kit
-
ELISA
-
Human
-
2.74~2000 pg/ml
Overview
Product Name
Human CCL3/MIP-1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.05 pg/ml (50 μl)；0.52 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0167 | 0.0161 | 0.0164 | |
| 2.74 | 0.0479 | 0.0428 | 0.0454 | 0.0290 |
| 8.23 | 0.0873 | 0.0832 | 0.0853 | 0.0689 |
| 24.69 | 0.2058 | 0.2010 | 0.2034 | 0.1870 |
| 74.07 | 0.6641 | 0.5979 | 0.6310 | 0.6146 |
| 222.22 | 1.4760 | 1.4170 | 1.4465 | 1.4301 |
| 666.67 | 2.5390 | 2.5340 | 2.5365 | 2.5201 |
| 2000.00 | 3.5360 | 3.5510 | 3.5435 | 3.5271 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 55.8 | 271.9 | 819.4 | 33.7 | 173.5 | 578.5 |
| Standard Deviation | 2.6 | 17.2 | 28.5 | 2.0 | 13.4 | 42.3 |
| Coefficient of Variation (%) | 4.7 | 6.3 | 3.5 | 6.0 | 7.7 | 7.3 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 CCL3/MIP-1，未加人 CCL3/MIP-1 的血清作为本底，计算回收率。回收率的范围从 94 % 至 114 %，平均回收率为 102%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 12.28-1051.89 | 100 | 224.55 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C C motif chemokine 3
CCL 3
CCL3
CCL3_HUMAN
Chemokine C C motif ligand 3
G0/G1 switch regulatory protein 19 1
G0/G1 switch regulatory protein 19-1
G0S19 1
G0S19 1 protein
Heparin binding chemotaxis protein
Expand