Human Leptin enzyme-linked immunoassay kit
Catalog# EH0056
Human Leptin enzyme-linked immunoassay kit
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ELISA
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Human
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40.96~10000 pg/ml
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unconjugated
Overview
Product Name
Human Leptin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
9.45 pg/ml (50 μl)；18.83 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0090 | 0.0090 | 0.0090 | |
| 40.96 | 0.0129 | 0.0136 | 0.0133 | 0.0043 |
| 102.40 | 0.0211 | 0.0208 | 0.0210 | 0.0120 |
| 256.00 | 0.0549 | 0.0544 | 0.0547 | 0.0457 |
| 640.00 | 0.2327 | 0.2207 | 0.2267 | 0.2177 |
| 1600.00 | 1.0060 | 0.9558 | 0.9809 | 0.9719 |
| 4000.00 | 2.7070 | 2.6740 | 2.6905 | 2.6815 |
| 10000.00 | 3.8850 | 3.8240 | 3.8545 | 3.8455 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 220.9 | 1012.4 | 3975.4 | 245.9 | 1135.2 | 4163.7 |
| Standard Deviation | 4.8 | 30.5 | 203.4 | 13.1 | 67.5 | 262.1 |
| Coefficient of Variation (%) | 2.2 | 3.0 | 5.1 | 5.3 | 5.9 | 6.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Leptin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 118% with an overall mean recovery of 104%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 393.86-7522.16 | 100 | 4332.2 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Leptin in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
FLJ94114
LEP
LEP_HUMAN
LEPD
Leptin (murine obesity homolog)
Leptin (obesity homolog
mouse)
Leptin
Leptin Murine Obesity Homolog
Leptin Precursor Obesity Factor
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