Human CD62E enzyme-linked immunoassay kit
Catalog# EH0198
Human CD62E enzyme-linked immunoassay kit
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ELISA
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Human
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8.23~6000 pg/ml
Overview
Product Name
Human CD62E enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.02 pg/ml (50 μl)；5.13 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0179 | 0.0184 | 0.0182 | |
| 8.23 | 0.0352 | 0.0343 | 0.0348 | 0.0166 |
| 24.69 | 0.0677 | 0.0684 | 0.0681 | 0.0499 |
| 74.07 | 0.1695 | 0.1696 | 0.1696 | 0.1514 |
| 222.22 | 0.4737 | 0.4691 | 0.4714 | 0.4533 |
| 666.67 | 1.2900 | 1.3210 | 1.3055 | 1.2874 |
| 2000.00 | 3.1610 | 3.2220 | 3.1915 | 3.1734 |
| 6000.00 | 4.4674 | 4.4727 | 4.4701 | 4.4519 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 163.0 | 726.7 | 2062.0 | 124.4 | 594.9 | 1727.9 |
| Standard Deviation | 9.2 | 29.1 | 128.8 | 5.2 | 31.9 | 74.36 |
| Coefficient of Variation (%) | 5.6 | 4.0 | 6.2 | 4.2 | 5.4 | 4.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD62E into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 115% with an overall mean recovery of 105%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 8.16-43.12 | 100 | 19.69 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD62E in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD26E
CD62 antigen-like family member E
CD62E
E selectin
E-selectin
ELAM
ELAM-1
ELAM1
Endothelial adhesion molecule 1
Endothelial leukocyte adhesion molecule 1
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