Human CD62E enzyme-linked immunoassay kit
Catalog# EH0198
Human CD62E enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
8.23~6000 pg/ml
Overview
Product Name
Human CD62E enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.02 pg/ml (50 μl)；5.13 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0179 | 0.0184 | 0.0182 | |
| 8.23 | 0.0352 | 0.0343 | 0.0348 | 0.0166 |
| 24.69 | 0.0677 | 0.0684 | 0.0681 | 0.0499 |
| 74.07 | 0.1695 | 0.1696 | 0.1696 | 0.1514 |
| 222.22 | 0.4737 | 0.4691 | 0.4714 | 0.4533 |
| 666.67 | 1.2900 | 1.3210 | 1.3055 | 1.2874 |
| 2000.00 | 3.1610 | 3.2220 | 3.1915 | 3.1734 |
| 6000.00 | 4.4674 | 4.4727 | 4.4701 | 4.4519 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 163.0 | 726.7 | 2062.0 | 124.4 | 594.9 | 1727.9 |
| Standard Deviation | 9.2 | 29.1 | 128.8 | 5.2 | 31.9 | 74.36 |
| Coefficient of Variation (%) | 5.6 | 4.0 | 6.2 | 4.2 | 5.4 | 4.3 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 CD62E，未加人 CD62E 的血清作为本底，计算回收率。回收率的范围从 85 % 至 115 %，平均回收率为 105 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 8.16-43.12 | 100 | 19.69 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD26E
CD62 antigen-like family member E
CD62E
E selectin
E-selectin
ELAM
ELAM-1
ELAM1
Endothelial adhesion molecule 1
Endothelial leukocyte adhesion molecule 1
ExpandFunction
E-选择素，也称为内皮白细胞粘附分子 1 (ELAM-1) 和白细胞-内皮细胞粘附分子 2 (LECAM-2)，已被指定为 CD62E。它是一种由活化内皮表达的细胞表面糖蛋白，介导血液中性粒细胞的粘附。