Human C-peptide enzyme-linked immunoassay kit
Catalog# EH0244
Human C-peptide enzyme-linked immunoassay kit
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ELISA
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Human
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7.81~500 pg/ml
Overview
Product Name
Human C-peptide enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.35 pg/ml (50 μl ) ；2.03 pg/ml (10 μl )
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0131 | 0.0140 | 0.0136 | |
| 7.81 | 0.0273 | 0.0295 | 0.0284 | 0.0149 |
| 15.63 | 0.0484 | 0.0472 | 0.0478 | 0.0343 |
| 31.25 | 0.0985 | 0.0893 | 0.0939 | 0.0804 |
| 62.50 | 0.2129 | 0.2179 | 0.2154 | 0.2019 |
| 125.00 | 0.5940 | 0.5637 | 0.5789 | 0.5653 |
| 250.00 | 1.5640 | 1.6090 | 1.5865 | 1.5730 |
| 500.00 | 3.2780 | 3.1740 | 3.2260 | 3.2125 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 8.48 | 57.94 | 164.31 | 11.5 | 54.5 | 164.1 |
| Standard Deviation | 0.60 | 2.74 | 6.73 | 0.9 | 1.2 | 5.0 |
| Coefficient of Variation (%) | 7.1 | 4.7 | 4.1 | 7.5 | 2.1 | 3.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human C-peptide into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 115% with an overall mean recovery of 108%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 228.33-2248.38 | 100 | 938.07 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of C-peptide in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃