Human Alpha-Synuclein enzyme-linked immunoassay kit
Catalog# EH0191
Human Alpha-Synuclein enzyme-linked immunoassay kit
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ELISA
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Human
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0.78~50 ng/ml
Overview
Product Name
Human Alpha-Synuclein enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.30 ng/ml (50 μl)；0.60 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0108 | 0.0105 | 0.0107 | |
| 0.78 | 0.0171 | 0.0166 | 0.0169 | 0.0062 |
| 1.56 | 0.0328 | 0.0294 | 0.0311 | 0.0205 |
| 3.13 | 0.0793 | 0.0817 | 0.0805 | 0.0699 |
| 6.25 | 0.2713 | 0.2822 | 0.2768 | 0.2661 |
| 12.50 | 1.0200 | 1.0220 | 1.0210 | 1.0104 |
| 25.00 | 3.0020 | 3.0060 | 3.0040 | 2.9934 |
| 50.00 | 4.5515 | 4.5446 | 4.5481 | 4.5374 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 3.1 | 8.6 | 22.3 | 2.7 | 7.8 | 20.3 |
| Standard Deviation | 0.1 | 0.2 | 0.9 | 0.1 | 0.2 | 0.5 |
| Coefficient of Variation (%) | 3.8 | 2.9 | 4.0 | 2.9 | 2.8 | 2.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Alpha-Synuclein into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 120% with an overall mean recovery of 99%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 126.53-645.86 | 100 | 269.28 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Alpha-Synuclein in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Alpha synuclein
Beta synuclein
NACP
Non A beta component of AD amyloid
PARK1
PARK4
PD1
SNCA
SNCB
Synuclein alpha
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