Human Alpha-Synuclein enzyme-linked immunoassay kit
Catalog# EH0191
Human Alpha-Synuclein enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
0.78~50 ng/ml
Overview
Product Name
Human Alpha-Synuclein enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.30 ng/ml (50 μl)；0.60 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0108 | 0.0105 | 0.0107 | |
| 0.78 | 0.0171 | 0.0166 | 0.0169 | 0.0062 |
| 1.56 | 0.0328 | 0.0294 | 0.0311 | 0.0205 |
| 3.13 | 0.0793 | 0.0817 | 0.0805 | 0.0699 |
| 6.25 | 0.2713 | 0.2822 | 0.2768 | 0.2661 |
| 12.50 | 1.0200 | 1.0220 | 1.0210 | 1.0104 |
| 25.00 | 3.0020 | 3.0060 | 3.0040 | 2.9934 |
| 50.00 | 4.5515 | 4.5446 | 4.5481 | 4.5374 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 3.1 | 8.6 | 22.3 | 2.7 | 7.8 | 20.3 |
| Standard Deviation | 0.1 | 0.2 | 0.9 | 0.1 | 0.2 | 0.5 |
| Coefficient of Variation (%) | 3.8 | 2.9 | 4.0 | 2.9 | 2.8 | 2.6 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 Alpha-Synuclein，未加人 Alpha-Synuclein 的血清作为本底，计算回收率。回收率的范围从 83 % 至 120 %，平均回收率为 99 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 126.53-645.86 | 100 | 269.28 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Alpha synuclein
Beta synuclein
NACP
Non A beta component of AD amyloid
PARK1
PARK4
PD1
SNCA
SNCB
Synuclein alpha
ExpandFunction
α-突触核蛋白 （SNCA） 是一种主要在中枢神经系统中表达的细胞质蛋白。它是淀粉样蛋白沉积中发现的一种次要蛋白质，是阿尔茨海默病的标志。SNCA 缺陷与家族性帕金森病有关。