Human Granzyme A/GZMA enzyme-linked immunoassay kit
Catalog# EH0129
Human Granzyme A/GZMA enzyme-linked immunoassay kit
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ELISA
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Human
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2.74~2000 pg/ml
Overview
Product Name
Human Granzyme A/GZMA enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.46 pg/ml (50 μl)；1.46 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0136 | 0.0141 | 0.0139 | |
| 2.74 | 0.0255 | 0.0239 | 0.0247 | 0.0109 |
| 8.23 | 0.0392 | 0.0410 | 0.0401 | 0.0263 |
| 24.69 | 0.0845 | 0.0824 | 0.0835 | 0.0696 |
| 74.07 | 0.2141 | 0.2112 | 0.2127 | 0.1988 |
| 222.22 | 0.6253 | 0.5911 | 0.6082 | 0.5944 |
| 666.67 | 1.7910 | 1.7440 | 1.7675 | 1.7537 |
| 2000.00 | 3.7830 | 3.7900 | 3.7865 | 3.7727 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 49.6 | 198.7 | 609.8 | 47.5 | 189.0 | 608.9 |
| Standard Deviation | 2.3 | 8.7 | 26.3 | 1.5 | 7.3 | 20.0 |
| Coefficient of Variation (%) | 4.6 | 4.4 | 4.3 | 3.2 | 3.9 | 3.3 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 Granzyme A，未加人 Granzyme A 的血清作为本底，计算回收率。回收率的范围从 81 % 至 97 %，平均回收率为 91 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 62.47-179.55 | 100.0 | 113.58 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CTL tryptase
CTLA3
Cytolytic t cell and natural killer cell specific trypsin like serine protease
Cytotoxic T lymphocyte associated serine esterase 3
Cytotoxic T lymphocyte proteinase 1
Cytotoxic T-lymphocyte proteinase 1
Fragmentin 1
Fragmentin-1
GRAA_HUMAN
Granzyme 1 cytotoxic T lymphocyte associated serine esterase 3
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