Human Lipoprotein A/LPA enzyme-linked immunoassay kit(one step)
Catalog# EHY0171
Human Lipoprotein A/LPA enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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1.37~1000 ng/ml
Overview
Product Name
Human Lipoprotein A/LPA enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.08 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0050 | 0.0051 | 0.0051 | |
| 1.37 | 0.0078 | 0.0088 | 0.0083 | 0.0033 |
| 4.12 | 0.0202 | 0.0171 | 0.0187 | 0.0136 |
| 12.35 | 0.0608 | 0.0575 | 0.0592 | 0.0541 |
| 37.04 | 0.1870 | 0.1751 | 0.1811 | 0.1760 |
| 111.11 | 0.5899 | 0.5499 | 0.5699 | 0.5649 |
| 333.33 | 1.4950 | 1.5180 | 1.5065 | 1.5015 |
| 1000.00 | 2.9860 | 2.8790 | 2.9325 | 2.9275 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 375.0 | 1408.8 | 3317.3 | 371.3 | 1405.8 | 3425.6 |
| Standard Deviation | 10.0 | 36.1 | 99.1 | 12.8 | 69.1 | 226.0 |
| Coefficient of Variation (%) | 2.7 | 2.6 | 3.0 | 3.4 | 4.9 | 6.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Lipoprotein A/LPA into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 118% with an overall mean recovery of 98%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 65.60-8958.87 | 100 | 2085.89 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Lipoprotein A/LPA in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
AK38
Antiangiogenic AK38 protein
apo(a)
APOA
apolipoprotein(a)
LP
lp(a)
LPA