Human TNF RII/TNFRSF1B enzyme-linked immunoassay kit
Catalog# EH0148
Human TNF RII/TNFRSF1B enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
5.49~4000 pg/ml
Overview
Product Name
Human TNF RII/TNFRSF1B enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.27 pg/ml (50 μl)；5.77 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0125 | 0.0118 | 0.0122 | |
| 5.49 | 0.0234 | 0.0224 | 0.0229 | 0.0108 |
| 16.46 | 0.0440 | 0.0410 | 0.0425 | 0.0304 |
| 49.38 | 0.1080 | 0.1087 | 0.1084 | 0.0962 |
| 148.15 | 0.2952 | 0.2944 | 0.2948 | 0.2827 |
| 444.44 | 0.8058 | 0.8283 | 0.8171 | 0.8049 |
| 1333.33 | 1.9330 | 1.9890 | 1.9610 | 1.9489 |
| 4000.00 | 3.6260 | 3.6600 | 3.6430 | 3.6309 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 77.5 | 375.0 | 1222.5 | 75.9 | 372.9 | 1201.0 |
| Standard Deviation | 4.4 | 18.1 | 69.5 | 4.2 | 16.8 | 26.2 |
| Coefficient of Variation (%) | 5.7 | 4.8 | 5.7 | 5.5 | 4.5 | 2.2 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 TNF RⅡ/TNFRSF1B，未加人 TNF RⅡ/TNFRSF1B 的血清作为本底，计算回收率。回收率的范围从 97 % 至 120 %，平均回收率为 115%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 24.09-59.68 | 100 | 34.98 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD120b
p75
p75 TNF receptor
p75TNFR
p80 TNF alpha receptor
p80 TNF-alpha receptor
Soluble TNFR1B variant 1
TBP-2
TBPII
TNF R II
ExpandFunction
TNF RII（肿瘤坏死因子受体 II），也称为 TNFRSF1B、p75/p80 和 CD120b，是一种广泛表达的膜相关 TNF-α 和淋巴毒素-α 受体。它的激活通过 NFkB 依赖性信号通路启动促炎和促存活反应，尽管它也可能诱导细胞凋亡。