Human CCL20/MIP-3 alpha enzyme-linked immunoassay kit
Catalog# EH0028
Human CCL20/MIP-3 alpha enzyme-linked immunoassay kit
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ELISA
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Human
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4.12~3000 pg/ml
Overview
Product Name
Human CCL20/MIP-3 alpha enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.72 pg/ml (50 μl) ； 0.89 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0096 | 0.0096 | 0.0096 | |
| 4.12 | 0.0226 | 0.0239 | 0.0233 | 0.0137 |
| 12.35 | 0.0538 | 0.0582 | 0.0560 | 0.0464 |
| 37.04 | 0.1590 | 0.1586 | 0.1588 | 0.1492 |
| 111.11 | 0.4436 | 0.4379 | 0.4408 | 0.4312 |
| 333.33 | 1.1440 | 1.2350 | 1.1895 | 1.1799 |
| 1000.00 | 2.4690 | 2.4030 | 2.4360 | 2.4264 |
| 3000.00 | 3.6820 | 3.7510 | 3.7165 | 3.7069 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 67.2 | 320.5 | 1005.9 | 58.9 | 310.9 | 1026.1 |
| Standard Deviation | 3.1 | 15.5 | 45.6 | 2.2 | 11.8 | 40.1 |
| Coefficient of Variation (%) | 4.6 | 4.8 | 4.5 | 3.7 | 3.8 | 3.9 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人CCL20，未加人 CCL20 的血清作为本底，计算回收率。回收率的范围从 81 % 至 119 %，平均回收率为 103 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 4.01-2566.88 | 100 | 606.71 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Beta chemokine exodus 1
Beta-chemokine exodus-1
C C motif chemokine ligand 20
C-C motif chemokine 20
CC chemokine LARC
Ccl20
CCL20(2-70)
CCL20_HUMAN
Chemokine (C C motif) ligand 20
Chemokine C-C motif chemokine 20
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