Human Complement factor B enzyme-linked immunoassay kit(one step)
Catalog# EHY0238
Human Complement factor B enzyme-linked immunoassay kit(one step)
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
46.88~3000 ng/ml
Overview
Product Name
Human Complement factor B enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.62 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0687 | 0.0675 | 0.0681 | |
| 46.88 | 0.1696 | 0.1820 | 0.1758 | 0.1077 |
| 93.75 | 0.2560 | 0.2624 | 0.2592 | 0.1911 |
| 187.50 | 0.4569 | 0.4582 | 0.4576 | 0.3895 |
| 375.00 | 0.7900 | 0.7890 | 0.7895 | 0.7214 |
| 750.00 | 1.4130 | 1.4390 | 1.4260 | 1.3579 |
| 1500.00 | 2.2060 | 2.2270 | 2.2165 | 2.1484 |
| 3000.00 | 2.8310 | 2.9550 | 2.8930 | 2.8249 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 85.84 | 349.12 | 1022.87 | 86.70 | 347.83 | 981.61 |
| Standard Deviation | 2.58 | 9.23 | 41.12 | 2.40 | 10.45 | 29.08 |
| Coefficient of Variation (%) | 3.0 | 2.6 | 4.0 | 2.8 | 3.0 | 3.0 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 Complement factor B，未加人 Complement factor B 的血清作为本底，计算回收率。回收率的范围从 80 % 至 119 %，平均回收率为 105%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
60 min
Standard Curve Gradient
7点
Incubation Count
2
Detectable Sample
Dosing Volume
10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C3/C5 convertase
Complement factor B
Glycine-rich beta glycoprotein
PBF2
Properdin factor B
Function
补体因子B是补体激活替代途径的一个组成部分。B因子以单链多肽的形式在血液中循环。在替代通路激活后，补体因子D将其切割，生成非催化链Ba和催化亚基Bb。活性亚基Bb是一种丝氨酸蛋白酶，与C3b结合形成替代通路C3转化酶。Bb参与预激活B淋巴细胞的增殖，而Ba则抑制其增殖。该基因定位于第6号染色体上主要组织兼容性复合体（MHC）III类区域。该基因簇包含多个参与免疫反应调控的基因。该基因的多腺苷酸化位点距离补体2组分基因的5'端421碱基对。