Human CD85d enzyme-linked immunoassay kit
Catalog# EH0046
Human CD85d enzyme-linked immunoassay kit
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ELISA
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Human
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312.5~20000 pg/ml
Overview
Product Name
Human CD85d enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
10.40 pg/ml (50 μl)：14.80 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0345 | 0.0329 | 0.0337 | |
| 312.50 | 0.0936 | 0.0922 | 0.0929 | 0.0592 |
| 625.00 | 0.1418 | 0.1472 | 0.1445 | 0.1108 |
| 1250.00 | 0.2482 | 0.2518 | 0.2500 | 0.2163 |
| 2500.00 | 0.4908 | 0.4994 | 0.4951 | 0.4614 |
| 5000.00 | 0.9079 | 0.9281 | 0.9180 | 0.8843 |
| 10000.00 | 1.8010 | 1.8280 | 1.8145 | 1.7808 |
| 20000.00 | 3.2730 | 3.2200 | 3.2465 | 3.2128 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 278.4 | 1676.4 | 5340.2 | 335.2 | 1676.4 | 5347.2 |
| Standard Deviation | 18.3 | 50.3 | 193.0 | 17.5 | 80.8 | 160.4 |
| Coefficient of Variation (%) | 6.6 | 3.0 | 3.6 | 5.2 | 4.8 | 3.0 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人CD85d，未加人 CD85d 的血清作为本底，计算回收率。回收率的范围从 80 % 至 127 %，平均回收率为 112 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 2.46-9.25 | 100 | 5.35 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD85 antigen-like family member D
CD85d
CD85d antigen
ILT 4
ILT-4
ILT4
Immunoglobulin like transcript 4
Immunoglobulin-like transcript 4
Leukocyte immunoglobulin like receptor 2
Leukocyte immunoglobulin like receptor subfamily B member 2
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