Human IL-1RA/IL-1F3 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

96T

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Catalog# EH0177

Human IL-1RA/IL-1F3 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

2.74~2000 pg/ml

Overview

Product Name

Human IL-1RA/IL-1F3 enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.09 pg/ml (50 μl)；0.15 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0625	0.0611	0.0618
2.74	0.0946	0.1014	0.0980	0.0362
8.23	0.1404	0.1366	0.1385	0.0767
24.69	0.2081	0.1979	0.2030	0.1412
74.07	0.3790	0.3737	0.3764	0.3146
222.22	0.9861	0.9935	0.9898	0.9280
666.67	2.1300	2.1710	2.1505	2.0887
2000.00	3.6470	3.7770	3.7120	3.6502

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	37.9	190.5	584.1	42.1	208.8	611.7
Standard Deviation	2.8	12.1	37.9	1.9	14.5	41.6
Coefficient of Variation (%)	7.4	6.3	6.5	5.3	4.2	4.6

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-1RA into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 109% with an overall mean recovery of 98%.

Sample Value

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	6.34-126.18	100	33.78

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-1RA in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: P18510 Human

Alias

DIRA

F630041P17Rik

ICIL 1RA

ICIL-1RA

ICIL1RA

IL-1ra

IL-1ra3

IL-1RN

IL1 inhibitor

IL1F3

Expand

Function

Interleukin-1 receptor antagonist (IL-1ra; also known as IL-1F3) is a 22-25 kDa member of the IL-1 family of cytokines. Currently, there are 11 family members (IL-1F1-F11), nine of which form an IL-1 gene cluster on human Ch2. Each IL-1 family member contains an IL-1 fold. This fold is generated by 12 packed beta -sheets that interact to form a beta -trefoil structure. Little amino acid (aa) homology is required to achieve this structure, and this explains the low aa identity among IL-1 family members. IL-1ra is a pure cytokine receptor antagonist that has no signal transduction-initiating activity. It is an acute phase protein that exists to dampen inflammation. IL-1( beta ) is initially produced by monocytes in response to a variety of stimuli. Circulating IL-1 then binds to widely expressed IL-1 type I receptors (IL-1 RI) and initiates a number of pro-inflammatory events. On endothelial cells (EC), IL-1 induces PGE2 and IL-6 release, generating fever, thrombocytosis, and hepatic acute phase protein production. In synovial joints, IL-1 induces chondrocyte NO production, an event that leads to reduced collagen synthesis and chondrocyte apoptosis. Finally, IL-1 increases neutrophil counts, both in blood and tissue, and thus is able to promote a pro-inflammatory environment in multiple locations. IL-1ra blocks IL-1 action through competitive inhibition. More correctly, although IL-1ra fills the IL-1 binding site in IL-1 RI, it is also unable to orchestrate the creation of a signal-transducing IL-1 RI:IL-1 R Accessory protein (IL-1 R AcP) heterodimer complex. Effective IL-1ra concentrations are generally 100-fold greater than local IL-1 concentrations. This is because the IL-1ra half-life is but 6 minutes, and very few IL-1 type I receptors need to be engaged by IL-1 to elicit a cellular response. Human IL-1ra is synthesized as a 177 aa precursor that contains a 25 aa signal sequence and a 152 aa mature region. Although it contains an IL-1 cytokine fold, it apparently lacks two structural motifs that allow for activation of the IL-1 receptor heterodimer. First, and following binding to IL-1 RI, the presence of Ile 51-His 54 and Lys 145 of the mature molecule preclude recruitment of IL-1 R AcP. Second, there is no identifiable C-terminal lectin segment that is hypothesized to help recruit an accessory signaling component. Mature human IL-1ra is 77% and 82% aa identical to mouse and canine IL-1ra, respectively, and human IL-1ra inhibits IL-1 activity on mouse cells. A number of cell types express IL-1ra, including monocytes, Sertoli cells, hepatocytes, adipocytes, synovial fibroblasts, mast cells, pancreatic beta -cells, and intestinal epithelial cells. There are at least three intracellular IL-1ra isoforms (icIL-1ra1, 2, and 3). All show N-terminal variation, and all contain amino acids 35-177 of the secreted precursor. Intracellular IL-1ra1 is of particular interest, because it is reported to be "secreted" by endothelial cells and binds to the IL-1 RI in an antagonist fashion. Intracellular IL-1ra1 is 159 aa in length and shows a 3 aa substitution for the first 21 aa's of the signal sequence of IL-1ra.

Human IL-1RA/IL-1F3 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Alias

Function

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