Human IL-2 Receptor alpha enzyme-linked immunoassay kit
Catalog# EH0170
Human IL-2 Receptor alpha enzyme-linked immunoassay kit
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ELISA
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Human
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1.37~1000 pg/ml
Overview
Product Name
Human IL-2 Receptor alpha enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.01 pg/ml (50 μl)；1.14 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0082 | 0.0086 | 0.0084 | |
| 1.37 | 0.0274 | 0.0256 | 0.0265 | 0.0181 |
| 4.12 | 0.0649 | 0.0683 | 0.0666 | 0.0582 |
| 12.35 | 0.1922 | 0.1804 | 0.1863 | 0.1779 |
| 37.04 | 0.4898 | 0.4806 | 0.4852 | 0.4768 |
| 111.11 | 1.2600 | 1.1500 | 1.2050 | 1.1966 |
| 333.33 | 2.4250 | 2.4020 | 2.4135 | 2.4051 |
| 1000.00 | 3.6340 | 3.6390 | 3.6365 | 3.6281 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 19.2 | 115.7 | 350.8 | 18.1 | 103.6 | 361.9 |
| Standard Deviation | 0.9 | 5.4 | 18.3 | 0.7 | 4.9 | 14.8 |
| Coefficient of Variation (%) | 4.7 | 4.7 | 5.2 | 3.9 | 4.7 | 4.1 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 IL-2 Receptor alpha，未加人 IL-2 Receptor alpha 的血清作为本底，计算回收率。回收率的范围从 89 % 至 118 %，平均回收率为 106 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 722.90-1772.29 | 100 | 1340.94 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Interleukin 2 receptor alpha chain
CD25
CD25 antigen
IDDM10
IL 2 receptor alpha subunit
IL-2 receptor subunit alpha
IL-2-RA
IL-2R subunit alpha
IL2 RA
IL2 Receptor alpha
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