Human MCP-2/CCL8 enzyme-linked immunoassay kit
Catalog# EH0277
Human MCP-2/CCL8 enzyme-linked immunoassay kit
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ELISA
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Human
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7.81~500 pg/ml
Overview
Product Name
Human MCP-2/CCL8 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
6.36 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0231 | 0.0254 | 0.0243 | |
| 7.81 | 0.0322 | 0.0239 | 0.0281 | 0.0038 |
| 15.63 | 0.0376 | 0.0297 | 0.0337 | 0.0094 |
| 31.25 | 0.0513 | 0.0457 | 0.0485 | 0.0243 |
| 62.50 | 0.1049 | 0.0956 | 0.1003 | 0.0760 |
| 125.00 | 0.2693 | 0.2709 | 0.2701 | 0.2459 |
| 250.00 | 0.8548 | 0.9259 | 0.8904 | 0.8661 |
| 500.00 | 2.7350 | 2.7400 | 2.7375 | 2.7133 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 12.4 | 51.1 | 155.6 | 11.9 | 51.3 | 151.7 |
| Standard Deviation | 0.88 | 2.72 | 4.81 | 0.9 | 1.5 | 4.1 |
| Coefficient of Variation (%) | 7.1 | 5.3 | 3.1 | 7.6 | 2.9 | 2.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human MCP-2/CCL8 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 79% to 117% with an overall mean recovery of 93%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 37.97-90.80 | 100 | 60.46 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human MCP-2/CCL8 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃