Human FGF basic /FGF2/bFGF enzyme-linked immunoassay kit
Catalog# EH0024
Human FGF basic /FGF2/bFGF enzyme-linked immunoassay kit
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ELISA
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Human
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8.19~2000 pg/ml
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unconjugated
Overview
Product Name
Human FGF basic /FGF2/bFGF enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.51 pg/ml (50 μl)；9.93 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0482 | 0.0491 | 0.0487 | |
| 8.19 | 0.0711 | 0.0715 | 0.0713 | 0.0227 |
| 20.48 | 0.1028 | 0.1063 | 0.1046 | 0.0559 |
| 51.20 | 0.1919 | 0.1881 | 0.1900 | 0.1414 |
| 128.00 | 0.4119 | 0.4048 | 0.4084 | 0.3597 |
| 320.00 | 0.9108 | 0.8764 | 0.8936 | 0.8450 |
| 800.00 | 1.9350 | 1.9990 | 1.9670 | 1.9184 |
| 2000.00 | 3.5980 | 3.6550 | 3.6265 | 3.5779 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 36.8 | 179.7 | 464.2 | 31.66 | 170.64 | 581.98 |
| Standard Deviation | 1.4 | 6.9 | 13.8 | 2.38 | 11.59 | 25.24 |
| Coefficient of Variation (%) | 3.9 | 3.8 | 3 | 7.5 | 6.8 | 4.3 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人FGF，未加人 FGF 的血清作为本底，计算回收率。回收率的范围从 78 % 至 92 %，平均回收率为 86 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.45-24.74 | 100 | 15.01 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 3 publications below |
Application in Publications
| Mouse | See 2 publications below |
| Human | See 1 publications below |
Target
UNIPROT
Alias
Basic fibroblast growth factor
Basic fibroblast growth factor bFGF
BFGF
FGF 2
FGF B
FGF-2
Fgf2
FGF2 basic
FGF2_HUMAN
FGFB
ExpandFunction
Citation
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Inhalable Chemotactic Liposome for Targeted Modulation of Pulmonary Pre-Metastatic Niche
Journal: Advanced Functional Materials
DOI:
IF: 18.5
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Jan
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Inhalable Chemotactic Liposome for Targeted Modulation of Pulmonary Pre-Metastatic Niche
Journal: Advanced Functional Materials
DOI: 10.1002/adfm.202419583
IF: 19
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Jan
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A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment
Journal: Bioactive Materials
DOI: 10.1016/j.bioactmat.2024.11.005
IF: 18
Application: ELISA
Reactivity: Human
Publish date: 2024 Dec