Human FGF basic /FGF2/bFGF enzyme-linked immunoassay kit
Catalog# EH0024
Human FGF basic /FGF2/bFGF enzyme-linked immunoassay kit
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ELISA
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Human
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8.19~2000 pg/ml
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unconjugated
Overview
Product Name
Human FGF basic /FGF2/bFGF enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.51 pg/ml (50 μl)；9.93 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0482 | 0.0491 | 0.0487 | |
| 8.19 | 0.0711 | 0.0715 | 0.0713 | 0.0227 |
| 20.48 | 0.1028 | 0.1063 | 0.1046 | 0.0559 |
| 51.20 | 0.1919 | 0.1881 | 0.1900 | 0.1414 |
| 128.00 | 0.4119 | 0.4048 | 0.4084 | 0.3597 |
| 320.00 | 0.9108 | 0.8764 | 0.8936 | 0.8450 |
| 800.00 | 1.9350 | 1.9990 | 1.9670 | 1.9184 |
| 2000.00 | 3.5980 | 3.6550 | 3.6265 | 3.5779 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 36.8 | 179.7 | 464.2 | 31.66 | 170.64 | 581.98 |
| Standard Deviation | 1.4 | 6.9 | 13.8 | 2.38 | 11.59 | 25.24 |
| Coefficient of Variation (%) | 3.9 | 3.8 | 3 | 7.5 | 6.8 | 4.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human FGF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 78% to 92% with an overall mean recovery of 86%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.45-24.74 | 100 | 15.01 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of HGF in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 3 publications below |
Application in Publications
| Mouse | See 2 publications below |
| Human | See 1 publications below |
Target
UNIPROT
Alias
Basic fibroblast growth factor
Basic fibroblast growth factor bFGF
BFGF
FGF 2
FGF B
FGF-2
Fgf2
FGF2 basic
FGF2_HUMAN
FGFB
ExpandFunction
Citation
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Inhalable Chemotactic Liposome for Targeted Modulation of Pulmonary Pre-Metastatic Niche
Journal: Advanced Functional Materials
DOI:
IF: 18.5
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Jan
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Inhalable Chemotactic Liposome for Targeted Modulation of Pulmonary Pre-Metastatic Niche
Journal: Advanced Functional Materials
DOI: 10.1002/adfm.202419583
IF: 19
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Jan
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A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment
Journal: Bioactive Materials
DOI: 10.1016/j.bioactmat.2024.11.005
IF: 18
Application: ELISA
Reactivity: Human
Publish date: 2024 Dec