Human IL-5 enzyme-linked immunoassay kit
Catalog# EH0008
Human IL-5 enzyme-linked immunoassay kit
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ELISA
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Human
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1.37~1000 pg/ml
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unconjugated
Overview
Product Name
Human IL-5 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.15 pg/ml (50 μl) ；0.73 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.0324 | 0.0303 | 0.0314 | |
| 1.37 | 0.0392 | 0.0346 | 0.0369 | 0.0056 |
| 4.12 | 0.0484 | 0.0466 | 0.0475 | 0.0162 |
| 12.35 | 0.0832 | 0.0818 | 0.0825 | 0.0512 |
| 37.04 | 0.1765 | 0.1662 | 0.1714 | 0.14 |
| 111.11 | 0.4454 | 0.4245 | 0.435 | 0.4036 |
| 333.33 | 1.1039 | 1.0797 | 1.0918 | 1.0605 |
| 1000 | 2.8645 | 2.8548 | 2.8597 | 2.8283 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 16.3 | 77.9 | 275.8 | 16.4 | 79.9 | 291.5 |
| Standard Deviation | 0.4 | 2.4 | 11.7 | 0.3 | 1.7 | 8.9 |
| Coefficient of Variation (%) | 2.7 | 3.1 | 4.2 | 2.1 | 2.1 | 3.1 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人IL-5，未加人 IL-5 的血清作为本底，计算回收率。
回收率的范围从 88 % 至 115 %，平均回收率为 106 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-6.72 | 21 | 2.13 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
B-cell differentiation factor I
Colony stimulating factor
EDF
Eosinophil differentiation factor
IL-5
IL5
IL5_HUMAN
Interleukin 5 (colony stimulating factor, eosinophil)
Interleukin 5
Interleukin-5
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