Human uPAR enzyme-linked immunoassay kit
Catalog# EH0077
Human uPAR enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
27.43~20000 pg/ml
Conjugation
-
unconjugated
Overview
Product Name
Human uPAR enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
8.79 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0148 | 0.0166 | 0.0157 | |
| 27.43 | 0.0262 | 0.0238 | 0.0250 | 0.0093 |
| 82.30 | 0.0453 | 0.0491 | 0.0472 | 0.0315 |
| 246.91 | 0.1061 | 0.1035 | 0.1048 | 0.0891 |
| 740.74 | 0.2701 | 0.2723 | 0.2712 | 0.2555 |
| 2222.22 | 0.7935 | 0.7777 | 0.7856 | 0.7699 |
| 6666.67 | 2.0410 | 2.0280 | 2.0345 | 2.0188 |
| 20000.00 | 3.9180 | 3.9010 | 3.9095 | 3.8938 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 370.1 | 1850.1 | 6148.8 | 356.9 | 1784.4 | 6135.0 |
| Standard Deviation | 10.2 | 87.9 | 204.1 | 18.4 | 88.2 | 389.3 |
| Coefficient of Variation (%) | 2.8 | 4.8 | 3.3 | 5.2 | 4.9 | 6.3 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人uPAR，未加人 uPAR 的血清作为本底，计算回收率。回收率的范围从 80 % 至 103%，平均回收率为 96 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1694.27-8807.19 | 100 | 3895.95 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD 87
CD87
CD87 antigen
MO 3
MO3
Monocyte activation antigen Mo3
Plasminogen activator receptor urokinase
Plasminogen activator urokinase receptor
PLAUR
U PAR
ExpandFunction
尿激酶型纤溶酶原激活受体 （uPAR） 是一种 GPI 锚定蛋白，可与非活性和活性形式的 uPA 结合。这种相互作用有助于定位和增强uPA的蛋白水解活性，导致纤溶酶原向纤溶酶的转化增加。uPA 还触发 uPAR 介导的信号转导、蛋白酪氨酸激酶的激活、基因表达、细胞粘附和趋化性。uPAR 还可以通过直接与玻连蛋白结合并阻止整合素与其配体结合来调节细胞粘附。