Human IL-17A enzyme-linked immunoassay kit
Catalog# EH0004
Human IL-17A enzyme-linked immunoassay kit
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ELISA
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Human
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1.37~1000 pg/ml
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unconjugated
Overview
Product Name
Human IL-17A enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.26 pg/ml (50 μl) ；0.65 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.0219 | 0.0209 | 0.0214 | |
| 1.37 | 0.0261 | 0.0261 | 0.0261 | 0.0047 |
| 4.12 | 0.0379 | 0.0395 | 0.0387 | 0.0173 |
| 12.35 | 0.0771 | 0.085 | 0.0811 | 0.0597 |
| 37.04 | 0.1799 | 0.1733 | 0.1766 | 0.1552 |
| 111.11 | 0.5178 | 0.5259 | 0.5219 | 0.5005 |
| 333.33 | 1.3929 | 1.4109 | 1.4019 | 1.3805 |
| 1000 | 3.2888 | 3.2794 | 3.2841 | 3.2627 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 27.6 | 113.6 | 265.5 | 23.9 | 125.5 | 324.1 |
| Standard Deviation | 0.9 | 5.3 | 11.6 | 0.8 | 4.2 | 8.2 |
| Coefficient of Variation (%) | 3.2 | 4.6 | 4.4 | 3.3 | 3.3 | 2.5 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人IL-17A，未加人 IL-17A 的血清作为本底，计算回收率。
回收率的范围从 80 % 至 112 %，平均回收率为 96 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-10.72 | 40 | 2.03 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CTLA 8
CTLA-8
CTLA8
cytotoxic T lymphocyte associated antigen 8
Cytotoxic T lymphocyte associated protein 8
Cytotoxic T lymphocyte associated serine esterase 8
Cytotoxic T-lymphocyte-associated antigen 8
IL 17
IL 17A
IL-17
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