Human IL-7 enzyme-linked immunoassay kit
Catalog# EH0045
Human IL-7 enzyme-linked immunoassay kit
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ELISA
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Human
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2.74~2000 pg/ml
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unconjugated
Overview
Product Name
Human IL-7 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.62 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0139 | 0.0144 | 0.0142 | |
| 2.74 | 0.0221 | 0.0202 | 0.0212 | 0.007 |
| 8.23 | 0.0347 | 0.0347 | 0.0347 | 0.021 |
| 24.69 | 0.0817 | 0.0730 | 0.0774 | 0.063 |
| 74.07 | 0.2122 | 0.1976 | 0.2049 | 0.191 |
| 222.22 | 0.6447 | 0.5979 | 0.6213 | 0.607 |
| 666.67 | 1.6810 | 1.5880 | 1.6345 | 1.620 |
| 2000.00 | 3.7070 | 3.5910 | 3.6490 | 3.635 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 40.2 | 198.2 | 606.4 | 41.1 | 221.2 | 654.7 |
| Standard Deviation | 2.1 | 11.2 | 19.1 | 2.4 | 10.6 | 21.7 |
| Coefficient of Variation (%) | 5.3 | 5.6 | 3.1 | 5.9 | 4.8 | 3.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-7 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 96% with an overall mean recovery of 88%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-30.80 | 25 | 10.94 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-8 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
IL 7
IL-7
Il7
IL7_HUMAN
Interleukin 7
Interleukin-7
LP-1
Lymphopoietin 1