Human TPO/Thrombopoietin enzyme-linked immunoassay kit
Catalog# EH0197
Human TPO/Thrombopoietin enzyme-linked immunoassay kit
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ELISA
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Human
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8.19~2000 pg/ml
Overview
Product Name
Human TPO/Thrombopoietin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.12 pg/ml (50 μl)；7.98 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0260 | 0.0252 | 0.0256 | |
| 8.19 | 0.0460 | 0.0467 | 0.0464 | 0.0208 |
| 20.48 | 0.0769 | 0.0723 | 0.0746 | 0.0490 |
| 51.20 | 0.1445 | 0.1427 | 0.1436 | 0.1180 |
| 128.00 | 0.3214 | 0.3069 | 0.3142 | 0.2886 |
| 320.00 | 0.6924 | 0.6953 | 0.6939 | 0.6683 |
| 800.00 | 1.6190 | 1.5460 | 1.5825 | 1.5569 |
| 2000.00 | 3.2320 | 3.2120 | 3.2220 | 3.1964 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 40.7 | 197.2 | 578.0 | 37.2 | 208.0 | 617.8 |
| Standard Deviation | 2.9 | 9.9 | 34.8 | 2.4 | 11.7 | 41.3 |
| Coefficient of Variation (%) | 7.1 | 5.0 | 6.0 | 6.5 | 5.6 | 6.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human TPO/Thrombopoietin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 128% with an overall mean recovery of 114%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.60-1005.88 | 100 | 253.32 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TPO/Thrombopoietin in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C mpl ligand
C-mpl ligand
Megakaryocyte colony stimulating factor
Megakaryocyte colony-stimulating factor
Megakaryocyte growth and development factor
Megakaryocyte stimulating factor
MGC163194
MGDF
MKCSF
ML
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