Human TPO/Thrombopoietin enzyme-linked immunoassay kit
Catalog# EH0197
Human TPO/Thrombopoietin enzyme-linked immunoassay kit
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ELISA
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Human
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8.19~2000 pg/ml
Overview
Product Name
Human TPO/Thrombopoietin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.12 pg/ml (50 μl)；7.98 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0260 | 0.0252 | 0.0256 | |
| 8.19 | 0.0460 | 0.0467 | 0.0464 | 0.0208 |
| 20.48 | 0.0769 | 0.0723 | 0.0746 | 0.0490 |
| 51.20 | 0.1445 | 0.1427 | 0.1436 | 0.1180 |
| 128.00 | 0.3214 | 0.3069 | 0.3142 | 0.2886 |
| 320.00 | 0.6924 | 0.6953 | 0.6939 | 0.6683 |
| 800.00 | 1.6190 | 1.5460 | 1.5825 | 1.5569 |
| 2000.00 | 3.2320 | 3.2120 | 3.2220 | 3.1964 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 40.7 | 197.2 | 578.0 | 37.2 | 208.0 | 617.8 |
| Standard Deviation | 2.9 | 9.9 | 34.8 | 2.4 | 11.7 | 41.3 |
| Coefficient of Variation (%) | 7.1 | 5.0 | 6.0 | 6.5 | 5.6 | 6.7 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 TPO/Thrombopoietin，未加人 TPO/Thrombopoietin 的血清作为本底，计算回收率。回收率的范围从 88 % 至 128 %，平均回收率为 114 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.60-1005.88 | 100 | 253.32 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C mpl ligand
C-mpl ligand
Megakaryocyte colony stimulating factor
Megakaryocyte colony-stimulating factor
Megakaryocyte growth and development factor
Megakaryocyte stimulating factor
MGC163194
MGDF
MKCSF
ML
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