Human TREM2 enzyme-linked immunoassay kit（one step）
Catalog# EHY0122
Human TREM2 enzyme-linked immunoassay kit（one step）
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ELISA
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Human
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0.14~100 ng/ml
Overview
Product Name
Human TREM2 enzyme-linked immunoassay kit（one step）
Product Type
Elisa Kit
Sensitivity
0.004 ng/ml (50 μl)；0.05 ng/ml (50 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0061 | 0.0054 | 0.0058 | |
| 0.14 | 0.0116 | 0.0115 | 0.0116 | 0.0058 |
| 0.41 | 0.0267 | 0.0281 | 0.0274 | 0.0217 |
| 1.23 | 0.0789 | 0.0792 | 0.0791 | 0.0733 |
| 3.70 | 0.2296 | 0.2361 | 0.2329 | 0.2271 |
| 11.11 | 0.6794 | 0.6593 | 0.6694 | 0.6636 |
| 33.33 | 1.7740 | 1.7290 | 1.7515 | 1.7458 |
| 100.00 | 3.3700 | 3.3340 | 3.3520 | 3.3463 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.9 | 10.3 | 35.3 | 2.0 | 10.0 | 32.2 |
| Standard Deviation | 0.1 | 0.4 | 1.8 | 0.1 | 0.5 | 1.9 |
| Coefficient of Variation (%) | 2.8 | 3.6 | 5.1 | 4.1 | 4.9 | 5.9 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 TREM2，未加人 TREM2的血清作为本底，计算回收率。回收率的范围从 95 % 至 105 %，平均回收率为 101 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.20-4.84 | 100.0 | 2.69 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
TREM 2
TREM-2
TREM2
TREM2_HUMAN
TREM2a
TREM2b
TREM2c
Trggering receptor expressed on myeloid cells 2
Trggering receptor expressed on myeloid cells 2a
Triggering receptor expressed on monocytes 2
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