Human FGF-21 enzyme-linked immunoassay kit
Catalog# EH0261
Human FGF-21 enzyme-linked immunoassay kit
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ELISA
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Human
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40.96~10000 pg/ml
Overview
Product Name
Human FGF-21 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
3.97 pg/ml (50 μl)；27.21 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0460 | 0.0428 | 0.0444 | |
| 40.96 | 0.0864 | 0.0780 | 0.0822 | 0.0378 |
| 102.40 | 0.1312 | 0.1391 | 0.1352 | 0.0908 |
| 256.00 | 0.2677 | 0.2552 | 0.2615 | 0.2171 |
| 640.00 | 0.5469 | 0.5421 | 0.5445 | 0.5001 |
| 1600.00 | 1.2130 | 1.1850 | 1.1990 | 1.1546 |
| 4000.00 | 2.4020 | 2.4170 | 2.4095 | 2.3651 |
| 10000.00 | 3.8410 | 3.8460 | 3.8435 | 3.7991 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 158.4 | 869.8 | 2834.0 | 157.6 | 862.8 | 2716.3 |
| Standard Deviation | 7.2 | 42.7 | 126.6 | 4.3 | 36.3 | 124.2 |
| Coefficient of Variation (%) | 4.6 | 4.9 | 4.5 | 2.7 | 4.2 | 4.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human FGF-21 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 120% with an overall mean recovery of 111%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| serum | 30 | 31.76-2828.07 | 100.0 | 864.79 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human FGF-21 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃