Human IL-10 enzyme-linked immunoassay kit

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Catalog# EH0009

Human IL-10 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

15.63~1000 pg/ml

Conjugation

unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Human IL-10 enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.35 pg/ml（50 μl）；0.61 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0268	0.0259	0.0264
15.63	0.0433	0.0397	0.0415	0.0152
31.25	0.0795	0.0831	0.0813	0.0550
62.50	0.1977	0.1791	0.1884	0.1621
125.00	0.4669	0.4558	0.4614	0.4350
250.00	1.1354	1.2026	1.1690	1.1427
500.00	2.7050	2.7878	2.7464	2.7201
1000.00	3.5502	3.5608	3.5555	3.5292

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	16.87	106.92	324.44	17.85	99.67	293.71
Standard Deviation	1.07	4.78	11.70	1.34	7.33	20.34
Coefficient of Variation (%)	6.3	4.5	3.6	7.5	7.4	6.9

板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。

板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。

Spike Recovery

健康人血清加入 3 个不同浓度水平的人IL-10，未加人 IL-10 的血清作为本底，计算回收率。

回收率的范围从 914% 至 109 %，平均回收率为 101 %。

Sample Value

Sample Matrix	Evaluated Sample	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	3.07-8.49	100	5.19

应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。

n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7点/3倍

Incubation Count

Detectable Sample

Dosing Volume

10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Application in Publications

ELISA

See 5 publications below

Application in Publications

Human

See 5 publications below

Target

UNIPROT

SwissProt: P22301 Human

Alias

CSIF

Cytokine synthesis inhibitory factor

GVHDS

IL 10

IL-10

IL10

IL10_HUMAN

IL10A

Interleukin 10

Interleukin-10

Expand

Function

白细胞介素-10 （IL-10），也称为细胞因子合成抑制因子（CSIF），是 IL-10 α-螺旋细胞因子家族的创始成员，该家族还包括 IL-19、IL-20、IL-22、IL-24 和 IL-26/AK155。IL-10由许多活化的造血细胞类型、肝星状细胞、角质形成细胞和胎盘细胞滋养层分泌。虽然人IL-10对小鼠细胞有活性，但小鼠IL-10不作用于人类细胞。成熟小鼠IL-10与大鼠IL-10共享85%的氨基酸序列同一性，与牛、犬、马、猫、人、绵羊和猪IL-10共享70-77%的氨基酸序列同一性。它包含两个链内二硫键，表达为36 kDa非共价连接的同源二聚体。 IL-10通过由II型细胞因子受体亚基IL-10 R α和IL-10 R β组成的异构受体复合物介导其生物活性。IL-10 R α 是一种 110 kDa 跨膜糖蛋白，在淋巴细胞、NK 细胞、巨噬细胞、单核细胞、星形胶质细胞、肠上皮细胞、细胞滋养层和活化的肝星状细胞上表达，而 75 kDa 跨膜 IL-10 R β 广泛表达。IL-10二聚体与两条IL 10 R α链结合，触发两条IL-10 R β链的募集。IL-10 R β不直接结合IL-10，但需要信号转导。IL-10 R β 还与 IL-20 R α、IL-22 R α 1 或 IL-28 R α 结合，形成 IL-22、IL-26、IL-28 和 IL-29 的受体复合物。 IL-10参与免疫调节包括抑制和刺激作用。它通过抑制Th1细胞和Th17细胞的扩增和活化以及促进M2巨噬细胞的发育来发挥抗炎细胞因子的作用。免疫抑制调节性T细胞（Treg）和调节性B细胞的表达对Treg增殖很重要。然而，在肿瘤微环境中，IL-10抑制Treg以及骨髓源来源性抑制细胞的扩增。IL-10诱导CD8+ T细胞的肿瘤内积累和活化。IL-10具有保护作用，包括限制关节炎炎症中的组织损伤和促进损伤后的肌肉再生，但它也有助于病毒感染的持续存在。干燥综合征（唾液）、原发性中枢神经系统淋巴瘤（脑脊液）和卵巢癌（血清和腹水）的 IL-10 水平升高。在复发性心脏病发作患者或先兆子痫期间的血清中，以及不育男性的中，其水平都会降低。

Citation

Filter by Application

Filter by Reactivity

miR-324-3p Drives Malignant Behaviors of Tumor Cells by Promoting M2 Macrophage Polarization in Laryngeal Squamous Cell Carcinoma
- Journal: Tohoku Journal Of Experimental Medicine
- DOI: 10.1620/tjem.2025.J083
- IF: 1.6
- Application: ELISA
- Reactivity: Human
- Publish date: 2026 Mar
MIR4435–2HG promotes breast cancer evolution through miR-205-5p/UBE2N axis and exosome-mediated macrophage M2-like polarization
- Journal: Cellular Signalling
- DOI: 10.1016/j.cellsig.2025.112287
- IF: 3.7
- Application: ELISA
- Reactivity: Human
- Publish date: 2025 Nov
Multi-omics insights of immune cells in the risk and prognosis of idiopathic membranous nephropathy
- Journal: Communications Biology
- DOI: 10.1038/s42003-025-08642-3
- IF: 5.1
- Application: ELISA
- Reactivity: Human
- Publish date: 2025 Aug
Indoleamine 2,3-dioxygenase 1 alters the proportions of B cell subpopulations in the microenvironment of acute myeloid leukemia
- Journal: Molecular Biomedicine
- DOI: 10.1186/s43556-025-00262-x
- IF: 6.3
- Application: ELISA
- Reactivity: Human
- Publish date: 2025 Apr
A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment
- Journal: Bioactive Materials
- DOI: 10.1016/j.bioactmat.2024.11.005
- IF: 18
- Application: ELISA
- Reactivity: Human
- Publish date: 2024 Dec

Human IL-10 enzyme-linked immunoassay kit

Usd:

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Application in Publications

Application in Publications

Target

UNIPROT

Alias

Function

Citation

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