Human PDGF-BB enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

96T

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Catalog# EH0068

Human PDGF-BB enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

27.43~20000 pg/ml

Overview

Product Name

Human PDGF-BB enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

7.56 pg/ml (50 μl)；2.79 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0089	0.0079	0.0084
27.43	0.0225	0.0201	0.0213	0.0129
82.30	0.0578	0.0504	0.0541	0.0457
246.91	0.1331	0.1487	0.1409	0.1325
740.74	0.3914	0.3825	0.3870	0.3786
2222.22	0.8560	0.8562	0.8561	0.8477
6666.67	1.5790	1.4540	1.5165	1.5081
20000.00	3.1680	3.0260	3.0970	3.0886

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	388.0	1692.3	5668.5	404.0	1977.6	5851.8
Standard Deviation	11.4	74.7	142.2	11.8	43.6	106.6
Coefficient of Variation (%)	6.0	4.4	5.3	5.8	4.5	3.7

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human PDGF-BB into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 91% to 112% with an overall mean recovery of 103%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	217.26-1712.20	100	608.29

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of PDGF-BB in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: P01127 Human

Alias

Becaplermin

c sis

FLJ12858

Oncogene SIS

PDGF 2

PDGF B chain

PDGF Beta

PDGF subunit B

PDGF-2

PDGF2

Expand

Function

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Human PDGF-BB enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Alias

Function

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