Human B7-H2/CD275 enzyme-linked immunoassay kit
Catalog# EH0133
Human B7-H2/CD275 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
13.72~10000 pg/ml
Overview
Product Name
Human B7-H2/CD275 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.01 pg/ml (50 μl)；4.95 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0144 | 0.0126 | 0.0135 | |
| 13.72 | 0.0336 | 0.0343 | 0.0340 | 0.0205 |
| 41.15 | 0.0807 | 0.0725 | 0.0766 | 0.0631 |
| 123.46 | 0.1894 | 0.2274 | 0.2084 | 0.1949 |
| 370.37 | 0.4951 | 0.5551 | 0.5251 | 0.5116 |
| 1111.11 | 1.2880 | 1.3600 | 1.3240 | 1.3105 |
| 3333.33 | 2.7260 | 2.8360 | 2.7810 | 2.7675 |
| 10000.00 | 4.1722 | 4.2258 | 4.1990 | 4.1855 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 246.6 | 1218.8 | 4221.3 | 132.7 | 829.4 | 3607.5 |
| Standard Deviation | 19.3 | 97.6 | 239.4 | 5.4 | 63.9 | 268.3 |
| Coefficient of Variation (%) | 7.8 | 8.0 | 5.7 | 4.0 | 7.7 | 7.4 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 B7-H2/CD275，未加人 B7-H2/CD275 的血清作为本底，计算回收率。回收率的范围从 72 % 至 107 %，平均回收率为 87 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.07-193.97 | 100 | 106.56 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
B7 H2
B7 homolog 2
B7 homologue 2
B7 like protein Gl50
B7 related protein 1
B7-H2
B7-like protein Gl50
B7-related protein 1
B7H2
B7RP 1
ExpandFunction
ICOSL蛋白（Inducible T-cell COStimulator Ligand），也称为B7h或CD275蛋白，是ICOS的配体，属于B7家族的成员之一。 ICOSL主要表达在抗原呈递细胞（如树突状细胞和B细胞）的表面。 ICOSL与ICOS的结合可以提供共刺激信号，促进T细胞的活化、增殖和分化。