Human CXCL5/ENA-78 enzyme-linked immunoassay kit
Catalog# EH0217
Human CXCL5/ENA-78 enzyme-linked immunoassay kit
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ELISA
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Human
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20.48~5000 pg/ml
Overview
Product Name
Human CXCL5/ENA-78 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.29 pg/ml (50 μl)；2.99 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0134 | 0.0136 | 0.0135 | |
| 20.48 | 0.0370 | 0.0376 | 0.0373 | 0.0238 |
| 51.20 | 0.0745 | 0.0774 | 0.0760 | 0.0625 |
| 128.00 | 0.1724 | 0.1706 | 0.1715 | 0.1580 |
| 320.00 | 0.4228 | 0.4138 | 0.4183 | 0.4048 |
| 800.00 | 1.0020 | 1.0140 | 1.0080 | 0.9945 |
| 2000.00 | 2.2430 | 2.1530 | 2.1980 | 2.1845 |
| 5000.00 | 3.9350 | 3.8410 | 3.8880 | 3.8745 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 135.3 | 659.5 | 1976.8 | 111.1 | 538.6 | 1723.8 |
| Standard Deviation | 8.7 | 37.6 | 102.5 | 4.3 | 17.4 | 78.9 |
| Coefficient of Variation (%) | 6.4 | 5.7 | 5.2 | 3.9 | 3.2 | 4.6 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 CXCL5/ENA-78 ，未加人 CXCL5/ENA-78 的血清作为本底，计算回收率。回收率的范围从 80 % 至 106 %，平均回收率为 89%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.93-7.91 | 100 | 3.83 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
AMCFII
C-X-C motif chemokine 5
C-X-C motif chemokine ligand 5
Chemokine (C X C motif) ligand 5
chemokine (C-X-C motif) ligand 5
Cxcl5
CXCL5_HUMAN
ENA 78
ENA-78 (8-78)
ENA-78(1-78)
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