Human MMP-3 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

96T

Bulk Order Quote | Customization Request

Catalog# EH0169

Human MMP-3 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

41.15~30000 pg/ml

Overview

Product Name

Human MMP-3 enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.79 ng/ml (50 μl)；4.66 ng/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0080	0.0084	0.0082
41.15	0.0319	0.0354	0.0337	0.0255
123.46	0.0831	0.0823	0.0827	0.0745
370.37	0.2282	0.2572	0.2427	0.2345
1111.11	0.6496	0.6786	0.6641	0.6559
3333.33	1.5680	1.6220	1.5950	1.5868
10000.00	2.9370	3.0260	2.9815	2.9733
30000.00	3.9709	4.0157	3.9933	3.9851

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	653.3	3162.9	11055.8	601.2	3117.6	9789.5
Standard Deviation	42.4	205.0	754.4	23.4	196.2	309.7
Coefficient of Variation (%)	6.5	6.5	6.8	3.9	6.3	3.2

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human MMP-3 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 107% with an overall mean recovery of 92%.

Sample Value

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	1.47-24.25	100	9.95

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of MMP-3 in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: P08254 Human

Alias

CHDS6

Matrix metalloproteinase 3

Matrix metalloproteinase-3

MGC126102

MGC126103

MGC126104

MMP 3

MMP-3

MMP3

MMP3_HUMAN

Expand

Function

Matrix metalloproteinases (MMPs), also called matrixins, constitute a family of zinc and calcium dependent endopeptidases that function in the breakdown of extracellular matrix (ECM). They play an important role in many normal physiological processes such as embryonic development, morphogenesis, reproduction and tissue remodeling. They also participate in many pathological processes such as arthritis, cancer and cardiovascular disease. While the amounts of newly synthesized MMPs are regulated mainly at the levels of transcription, the proteolytic activities of existing MMPs are controlled through both the activation of proenzymes or zymogens and the inhibition of active enzymes by endogenous inhibitors, alpha -macroglobulins and tissue inhibitors of metalloproteinases (TIMPs). MMP-3 (also referred to as stromelysin-1) may be expressed in fibroblasts, chondrocytes, endothelial cells, macrophages, vascular smooth muscle cells, osteoblasts, and keratinocytes in response to appropriate stimuli. Various agents regulate its biosynthesis. Inflammatory cytokines such as IL-1 and TNF-alpha, epidermal growth factor, platelet-derived growth factor, phorbol and oncogenic cellular transformation are the inductive agents. In comparison, retinoic acid, glucocorticoids, estrogen, progesterone and TGF-beta suppress MMP-3 synthesis. MMP-3 is secreted from the cells as a proenzyme. The proenzyme has been shown to stimulate plasminogen activation. The N-terminal pro-domain contains the cysteine switch motif conserved in MMPs that maintains MMP-3 in the latent state. Activation of the proenzyme results in the removal of the pro-domain. MMP-3 activation can be achieved in vitro by proteases such as itself, chyrotrypsin, neutrophil elastase and plasma kallikrein, and by mercury compounds. The resulting active enzyme consists of a catalytic domain with a zinc-binding motif conserved in metzincins. A short hinge peptide links the catalytic domain to the C-terminal hemopexin-like domain. The active MMP-3 is capable of cleaving types III, IV, IX and X collagen, aggrecan, fibronectin, laminin, IGFBP-3, serpins, and IL-1 beta. The active enzyme also activates proMMP-1, -8, -9, and -13. Therefore, it is suggested that MMP-3 may participate in physiological matrix turnover and pathological destruction of the tissue. For example, MMP-3 is required for the generation of a macrophage chemoattractant in a model of herniated disc resorption.

Human MMP-3 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Alias

Function

Same Target and Pathway Products

Human/Mouse MASP2 enzyme-linked immunoassay kit

Human IL-6 Super Sensitivity enzyme-linked immunoassay kit

Human Complement factor B enzyme-linked immunoassay kit(one step)

Human SNAP25 enzyme-linked immunoassay kit

Human CX3CL1/Fractalking enzyme-linked immunoassay kit

Human tPA enzyme-linked immunoassay kit(one step)

Human FGF-19 enzyme-linked immunoassay kit

Human Neuropilin-1/NRP1 enzyme-linked immunoassay kit（one step）

Human/Monkey Complement C3 enzyme-linked immunoassay kit(one step)

Human CD48 enzyme-linked immunoassay kit

Human/Monkey Albumin enzyme-linked immunoassay kit(one step)

Human MMP-7 enzyme-linked immunoassay kit

Human CTLA-4 enzyme-linked immunoassay kit

Human/Monkey/Canine CCL5/RANTES enzyme-linked immunoassay kit(one step)

Human MERTK enzyme-linked immunoassay kit（one step）

Human Lipoprotein A/LPA enzyme-linked immunoassay kit(one step)

Human CTGF/CCN2 enzyme-linked immunoassay kit(one step)

Human Proprotein Convertase 9/PCSK9 enzyme-linked immunoassay kit（one step）

Lentivirus HIV-1 p24 enzyme-linked immunoassay kit（one step）

Human CCL2/MCP-1 enzyme-linked immunoassay kit

Human CCL5/RANTES enzyme-linked immunoassay kit

Human Antithrombin III/AT III enzyme-linked immunoassay kit(one step)

Human CD273/B7-DC/PD-L2 enzyme-linked immunoassay kit（one step）

Human CD73 enzyme-linked immunoassay kit

Human ICAM-1 / CD54 enzyme-linked immunoassay kit

Human CD47 enzyme-linked immunoassay kit

Human IL-17E/IL-25 enzyme-linked immunoassay kit

Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit

Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit

Human Total HSP60 enzyme-linked immunoassay kit

Human IL-13R alpha 2 enzyme-linked immunoassay kit

Human CHIT1 enzyme-linked immunoassay kit

Human RBP4 enzyme-linked immunoassay kit（one step）

Human Caspase-9 enzyme-linked immunoassay kit

Human Granzyme A/GZMA enzyme-linked immunoassay kit

Human IFN-β enzyme-linked immunoassay kit

Human ULBP1/NKG2DL1 enzyme-linked immunoassay kit

Human CD163 enzyme-linked immunoassay kit

Human Renin enzyme-linked immunoassay kit

Human CXCL2/MIP2/GRO beta enzyme-linked immunoassay kit

Human SAA1 enzyme-linked immunoassay kit

Human IL-3 enzyme-linked immunoassay kit

Human/Monkey PCSK9 enzyme-linked immunoassay kit (one step)

Human TGF-α enzyme-linked immunoassay kit

Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)

Human Leptin enzyme-linked immunoassay kit

Human CD27/TNFRSF7 enzyme-linked immunoassay kit

Human B7-H6/NCR3LG1 enzyme-linked immunoassay kit

Human/Monkey/Canine IL-18 enzyme-linked immunoassay kit

Human Endoglin/CD105 enzyme-linked immunoassay kit

Human IL-1RA/IL-1F3 enzyme-linked immunoassay kit

Human beta-NGF enzyme-linked immunoassay kit

Human IL-15 enzyme-linked immunoassay kit

Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step)

Human CD28 enzyme-linked immunoassay kit