Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step)
Catalog# EHY0160
Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.05~40 ng/ml
Overview
Product Name
Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
2.18 pg/ml (50 μl)；40.00 pg/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0073 | 0.0071 | 0.0072 | |
| 0.05 | 0.0128 | 0.0128 | 0.0128 | 0.0056 |
| 0.16 | 0.0240 | 0.0223 | 0.0232 | 0.0160 |
| 0.49 | 0.0578 | 0.0546 | 0.0562 | 0.0490 |
| 1.48 | 0.1659 | 0.1523 | 0.1591 | 0.1519 |
| 4.44 | 0.5147 | 0.4948 | 0.5048 | 0.4976 |
| 13.33 | 1.5870 | 1.5630 | 1.5750 | 1.5678 |
| 40.00 | 3.8260 | 3.8630 | 3.8445 | 3.8373 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 0.9 | 4.1 | 12.3 | 0.8 | 3.8 | 12.2 |
| Standard Deviation | 0.0 | 0.1 | 0.4 | 0.0 | 0.2 | 0.3 |
| Coefficient of Variation (%) | 3.1 | 2.8 | 3.0 | 4.7 | 4.2 | 2.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human PAI-1/Serpin E1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 101% with an overall mean recovery of 94%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 98.03-475.14 | 100 | 264.19 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of PAI-1/Serpin E1 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Clade E
Endothelial plasminogen activator inhibitor
Nexin
Nexin plasminogen activator inhibitor type 1
PAI 1
PAI
PAI-1
PAI1_HUMAN
PLANH1
Plasminogen activator inhibitor 1
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