Human CXCL9/MIG enzyme-linked immunoassay kit
Catalog# EH0015
Human CXCL9/MIG enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
6.86~5000 pg/ml
Overview
Product Name
Human CXCL9/MIG enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.24 pg/ml (50 μl)；5.32 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0162 | 0.0167 | 0.0165 | |
| 6.86 | 0.0222 | 0.0225 | 0.0224 | 0.0059 |
| 20.58 | 0.0352 | 0.0373 | 0.0363 | 0.0198 |
| 61.73 | 0.0766 | 0.0728 | 0.0747 | 0.0583 |
| 185.19 | 0.2182 | 0.2146 | 0.2164 | 0.2000 |
| 555.56 | 0.7838 | 0.7383 | 0.7611 | 0.7446 |
| 1666.67 | 2.4443 | 2.4961 | 2.4702 | 2.4538 |
| 5000.00 | 4.1092 | 4.1881 | 4.1487 | 4.1322 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 76.4 | 387.0 | 1421.7 | 78.7 | 376.7 | 1375.0 |
| Standard Deviation | 2.1 | 11.4 | 34.2 | 6.0 | 8.6 | 31.7 |
| Coefficient of Variation (%) | 2.8 | 3.0 | 2.4 | 7.6 | 2.3 | 2.3 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人CXCL9，未加人 CXCL9 的血清作为本底，计算回收率。回收率的范围从 83 % 至 97 %，平均回收率为 91 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-196.43 | 92 | 31.69 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C-X-C motif chemokine 9
chemokine (C-X-C motif) ligand 9
CMK
crg-10
CXCL9
CXCL9_HUMAN
gamma interferon induced monokine
Gamma-interferon-induced monokine
HuMIG
MIG
ExpandFunction
CXCL9，也称为MIG，是一种趋化因子，在巨噬细胞和中枢神经系统神经胶质细胞中诱导IFN-γ。CXCL9 通过 CXCR3 发出信号，诱导活化 T 细胞和肿瘤浸润淋巴细胞的化学吸引。