Human GITR/TNFRSF18 enzyme-linked immunoassay kit
Catalog# EH0095
Human GITR/TNFRSF18 enzyme-linked immunoassay kit
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ELISA
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Human
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5.49~4000 pg/ml
Overview
Product Name
Human GITR/TNFRSF18 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.07 pg/ml (50 μl)；1.20 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0145 | 0.0146 | 0.0146 | |
| 5.49 | 0.0260 | 0.0256 | 0.0258 | 0.0113 |
| 16.46 | 0.0492 | 0.0497 | 0.0495 | 0.0349 |
| 49.38 | 0.1206 | 0.1200 | 0.1203 | 0.1058 |
| 148.15 | 0.3372 | 0.3175 | 0.3274 | 0.3128 |
| 444.44 | 0.9332 | 0.9130 | 0.9231 | 0.9086 |
| 1333.33 | 2.4945 | 2.4639 | 2.4792 | 2.4647 |
| 4000.00 | 4.1397 | 4.2936 | 4.2167 | 4.2021 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 1410.0 | 535.3 | 111.0 | 1556.0 | 680.1 | 136.7 |
| Standard Deviation | 55.5 | 10.1 | 2.4 | 102.5 | 32.1 | 9.1 |
| Coefficient of Variation (%) | 3.9 | 1.9 | 2.2 | 6.6 | 4.7 | 6.7 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人GITR/TNFRSF18，未加人 GITR/TNFRSF18的血清作为本底，计算回收率。回收率的范围从 84 % 至 111 %，平均回收率为 98 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-1.19 | 75 | 0.47 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Activation inducible TNFR family receptor
Activation-inducible TNFR family receptor
AITR
CD357
GITR
GITR D
GITR-D
GITRD
Glucocorticoid induced TNFR related protein
glucocorticoid-induced tnf receptor ligand
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